Schiffman G, Douglas R M, Bonner M J, Robbins M, Austrian R
J Immunol Methods. 1980;33(2):133-44. doi: 10.1016/s0022-1759(80)80004-4.
A radioimmunossay is described which uses a 14C biosynthetically internally labeled antigen. This modified Farr technique has been standardized by quantitative precipitation and compared with hemagglutination and mouse protection. Specificity was established by use of heterologous hyperimmune sera and by use of unlabeled pneumococcal polysaccharides for inhibition. Reproducibility has been evaluated for different preparations of antigens and varying storage conditions of sera. The system has been applied to a wide variety of studies requiring analysis of human and animal sera.
本文描述了一种使用经生物合成内部标记有¹⁴C的抗原的放射免疫测定法。这种改良的法尔技术已通过定量沉淀进行了标准化，并与血凝反应和小鼠保护试验进行了比较。通过使用异种超免疫血清以及使用未标记的肺炎球菌多糖进行抑制来确定特异性。已针对不同的抗原制剂和血清的不同储存条件评估了重现性。该系统已应用于需要分析人血清和动物血清的各种研究。
