Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic.
Department of Microelectronics, Brno University of Technology, Brno, Czech Republic.
PLoS One. 2019 Mar 22;14(3):e0214066. doi: 10.1371/journal.pone.0214066. eCollection 2019.
Selenium nanoparticle modified surfaces attract increasing attention in the field of tissue engineering. Selenium exhibits strong anticancer, antibacterial and anti-inflammatory properties and it maintains relatively low off-target cytotoxicity. In our paper, we present the fabrication, characterization and cytocompatibility of titanium oxide (TiO2) nanotube surface decorated with various surface densities of chemically synthesized selenium nanoparticles. To evaluate antibacterial and anti-cancer properties of such nanostructured surface, gram negative bacteria E. coli, cancerous osteoblast like MG-63 cells and non-cancerous fibroblast NIH/3T3 were cultured on designed surfaces. Our results suggested that selenium nanoparticles improved antibacterial properties of titanium dioxide nanotubes and confirmed the anticancer activity towards MG-63 cells, with increasing surface density of nanoparticles. Further, the selenium decorated TiO2 nanotubes suggested deteriorating effect on the cell adhesion and viability of non-cancerous NIH/3T3 cells. Thus, we demonstrated that selenium nanoparticles decorated TiO2 nanotubes synthesized using sodium selenite and glutathione can be used to control bacterial infections and prevent the growth of cancerous cells. However, the higher surface density of nanoparticles adsorbed on the surface was found to be cytotoxic for non-cancerous NIH/3T3 cells and thus it might complicate the integration of biomaterial into the host tissue. Therefore, an optimal surface density of selenium nanoparticles must be found to effectively kill bacteria and cancer cells, while remaining favorable for normal cells.
纳米硒粒子修饰表面在组织工程领域受到越来越多的关注。硒具有很强的抗癌、抗菌和抗炎特性，且具有相对较低的脱靶细胞毒性。在我们的论文中，我们介绍了通过化学合成的纳米硒粒子修饰氧化钛（TiO2）纳米管表面的制备、表征和细胞相容性。为了评估这种纳米结构表面的抗菌和抗癌特性，将革兰氏阴性菌大肠杆菌、癌性成骨细胞样 MG-63 细胞和非癌性成纤维细胞 NIH/3T3 培养在设计的表面上。我们的结果表明，硒纳米粒子提高了二氧化钛纳米管的抗菌性能，并证实了对 MG-63 细胞的抗癌活性，随着纳米粒子表面密度的增加而增强。此外，硒修饰的 TiO2 纳米管对非癌性 NIH/3T3 细胞的细胞黏附和活力有恶化作用。因此，我们证明了使用亚硒酸钠和谷胱甘肽合成的硒纳米粒子修饰的 TiO2 纳米管可用于控制细菌感染和防止癌细胞生长。然而，吸附在表面上的更高表面密度的纳米粒子对非癌性 NIH/3T3 细胞具有细胞毒性，因此可能会使生物材料与宿主组织的整合复杂化。因此，必须找到最佳的硒纳米粒子表面密度，以有效地杀死细菌和癌细胞，同时对正常细胞保持有利。
