嗅神经通路损伤对派伊尔氏结肠道免疫缺陷的靶向作用：首个实验研究。

Mysterious effects of olfactory pathway lesions on intestinal immunodeficiency targeting Peyer's patches: The first experimental study.

机构信息

Ataturk University, Medical Faculty, Department of Pediatric Surgery, Erzurum, Turkey.

Ataturk University, Medical Faculty, Department of General Surgery, Erzurum, Turkey.

出版信息

Med Hypotheses. 2019 Apr;125:31-36. doi: 10.1016/j.mehy.2019.02.032. Epub 2019 Feb 11.

DOI:10.1016/j.mehy.2019.02.032

PMID:30902148

Abstract

BACKGROUND

Although olfaction has been considered as important neuroimmunomodulatory foundation, there is no satisfying analytical information between neurohistomorphological features olfactory networks and intestinal immune system hardwares. We studied if the olfactory bulb lesions (OBL) may rely on histopathological features of intestinal lymphatic Peyer's patches in an animal model.

METHODS

Thirty-two rats were grouped as control (Group I, n = 8), SHAM (Group II, n = 7) and OBL (Group III, n = 17) respectively; and followed eight weeks and animals were decapitated. The olfactory bulbs and intestines were extracted. Specimens stained with hematoxylin/eosin and GFAP methods and analyzed Stereologically to evaluate volume loss of olfactory bulbs and Peyer's patches volumes (PV) of intestines per cubic millimeter and compared with each other's statistically.

RESULTS

The mean olfactory bulbs volumes were estimated as 3.65 ± 0.32/mm in group I, 3.12 ± 0.20/mm in group II and 2.21 ± 0.15/mm in group III (p < 0.0005 Group III vs. I and II). The mean of PV were estimated as; (9 ± 2) × 10 µm/cm in Group-I, (12 ± 3) × 10 µm/cm in Group-II; and (23 ± 4) × 10 µm/cm in group-III (p < 0.005 Group II vs. I, p < 0.0005 Group III vs. I-II).

CONCLUSIONS

OBL could rely on intestinal immunodeficiency causing by olfaction loss induced denervation injury of Peyer's patches.

摘要

背景

尽管嗅觉已被认为是重要的神经免疫调节基础，但神经组织形态学特征嗅觉网络与肠道免疫系统硬件之间没有令人满意的分析信息。我们研究了嗅球病变（OBL）是否可以依赖于动物模型中肠道淋巴派尔集合淋巴结的组织病理学特征。

方法

32 只大鼠分为对照组（I 组，n=8）、假手术组（II 组，n=7）和 OBL 组（III 组，n=17）；并分别随访 8 周，然后处死动物。提取嗅球和肠道标本。用苏木精/伊红和 GFAP 方法染色，并通过体视学分析评估嗅球体积损失和肠道派尔集合淋巴结体积（PV）每立方毫米，并进行统计学比较。

结果

I 组嗅球平均体积估计为 3.65±0.32/mm，II 组为 3.12±0.20/mm，III 组为 2.21±0.15/mm（p<0.0005 III 组与 I 组和 II 组相比）。I 组 PV 平均估计值为；（9±2）×10µm/cm，II 组为（12±3）×10µm/cm；III 组为（23±4）×10µm/cm（p<0.005 II 组与 I 组相比，p<0.0005 III 组与 I-II 组相比）。