Saxena S K, Thompson J S, Sharp J G
Department of Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, USA.
J Invest Surg. 1997 May-Jun;10(3):97-103. doi: 10.3109/08941939709032139.
Intestinal lymphoid tissue has a complex interrelationship with the epithelium. The epithelia of intestinal crypts associated with lymphoid aggregates have an increased proliferation rate. In the present study, the authors tested the hypothesis that organized intestinal lymphoid tissue (Peyer's patches) enhances intestinal regeneration by studying this process with and without an adjacent Peyer's patch. Forty adult male Sprague-Dawley rats had full-thickness ileal defects patched with cecal serosa to allow regeneration of ileal mucosa. Control animals (group I) had the patch constructed adjacent to a Peyer's patch, whereas this Peyer's patch was excised in group II. Intestinal regeneration in both groups was evaluated on the third, fifth, seventh, and ninth days after operation. During the early phase of regeneration, both epithelial cell proliferation and migration were decreased in the patched defect after excision of the Peyer's patch. Crypt cell production rate in the adjacent normal mucosa also was decreased after excision of the Peyer's patch. Excision of the Peyer's patch resulted in less well-developed crypts and villi. Wound contraction, however, was greater in the intestinal defect adjacent to the Peyer's patch until day 7. In conclusion, Peyer's patches have a facilitative effect on the healing of intestinal wounds by promoting both epithelial cell migration on the defect and epithelial cell proliferation in the crypts adjacent to the wound and by decreasing the rate of wound contraction. These findings support a role for intestinal lymphoid tissue in the regulation of epithelial cell maintenance.
肠道淋巴组织与上皮细胞有着复杂的相互关系。与淋巴集结相关的肠隐窝上皮细胞增殖率增加。在本研究中，作者通过研究有无相邻派尔集合淋巴结的情况下的这一过程，验证了有组织的肠道淋巴组织（派尔集合淋巴结）可促进肠道再生的假说。40只成年雄性Sprague-Dawley大鼠，用盲肠浆膜修补全层回肠缺损，以促进回肠黏膜再生。对照组动物（I组）在派尔集合淋巴结附近进行修补，而在II组中将该派尔集合淋巴结切除。在术后第3、5、7和9天评估两组的肠道再生情况。在再生早期，切除派尔集合淋巴结后，修补缺损处的上皮细胞增殖和迁移均减少。切除派尔集合淋巴结后，相邻正常黏膜中的隐窝细胞产生率也降低。派尔集合淋巴结的切除导致隐窝和绒毛发育不良。然而，直到第7天，靠近派尔集合淋巴结的肠道缺损处的伤口收缩更大。总之，派尔集合淋巴结通过促进缺损处上皮细胞迁移和伤口相邻隐窝中的上皮细胞增殖以及降低伤口收缩率，对肠道伤口愈合具有促进作用。这些发现支持肠道淋巴组织在调节上皮细胞维持方面的作用。
