College of Veterinary Medicine, Hebei Agricultural University, Baoding, Hebei 071000, China.
College of Veterinary Medicine, Hebei Agricultural University, Baoding, Hebei 071000, China.
Food Chem. 2019 Aug 1;288:347-353. doi: 10.1016/j.foodchem.2019.03.031. Epub 2019 Mar 12.
The objective of this study is to report a molecularly imprinted polymer-based chemiluminescence method for determination of Sudan dyes. A dummy-template molecularly imprinted polymer capable of recognizing seven Sudan dyes was first synthesized and its recognition mechanism was studied by using computation simulation method. The polymer was coated in the wells of conventional microplate to prepare a chemiluminescence sensor and the assay process consisted of only one sample-loading step prior to signal acquisition. The optimized sensor was used to determine the seven dyes in egg yolk and the results were confirmed with a high performance liquid chromatography. Results showed that this sensor achieved ultrahigh sensitivity (1.0-5.0 pg/mL), rapid assay process (10 min) and satisfactory recovery (70.5%-92.2%). Furthermore, the sensor could be reused for 5 times. Therefore, this sensor could be used as a useful tool for screening the residues of Sudan dyes in egg.
本研究旨在报道一种基于分子印迹聚合物的化学发光法用于苏丹染料的测定。首先合成了一种能够识别七种苏丹染料的假模板分子印迹聚合物，并通过计算模拟方法研究了其识别机制。将聚合物涂覆在常规微孔板的孔中，制备化学发光传感器，测定过程仅在信号采集前进行一次样品加载步骤。优化后的传感器用于测定蛋黄中的七种染料，并通过高效液相色谱法进行了验证。结果表明，该传感器具有超高的灵敏度（1.0-5.0 pg/mL）、快速的测定过程（10 min）和令人满意的回收率（70.5%-92.2%）。此外，该传感器可重复使用 5 次。因此，该传感器可作为一种有用的工具，用于筛选鸡蛋中苏丹染料的残留。
