Xia Wan Qiu, Huang Jun, Wang Geng Nan, Liu Jing, Wang Jian Ping
College of Veterinary Medicine, Hebei Agricultural University, Baoding, Hebei, 071000, China.
Continuing Education College, Hebei Agricultural University, Baoding, Hebei, 071000, China.
Anal Biochem. 2018 Aug 1;554:9-15. doi: 10.1016/j.ab.2018.05.021. Epub 2018 May 25.
In this study, a molecularly imprinted polymer based chemiluminescence array capable of simultaneous determining phenothiazines and benzodiazepines was first reported. Two polymers were coated in different wells of the conventional 96-well microtiter plate as the recognition reagents, and the added analytes competed with a horseradish peroxidase-labeled bi-hapten conjugate to bind the recognition reagents. The light signal was induced by using a highly effective luminol-HO-IMP system. The assay procedure consisted of only one sample-loading step prior to data acquisition. Then, the array was used to determine 4 phenothiazines and 5 benzodiazepines in pork simultaneously. The limits of detection for the 9 drugs were in a range of 0.001-0.01 ng/mL, and the recoveries from the fortified blank pork were in a range of 63.5%-94.1%. Furthermore, the array could be reused for 8 times. The detection results for some real pork samples were consistent with an ultra performance liquid chromatography method.
在本研究中，首次报道了一种基于分子印迹聚合物的化学发光阵列，能够同时测定吩噻嗪类药物和苯二氮䓬类药物。将两种聚合物包被在传统96孔微量滴定板的不同孔中作为识别试剂，加入的分析物与辣根过氧化物酶标记的双半抗原缀合物竞争结合识别试剂。使用高效的鲁米诺-H₂O₂体系诱导光信号。检测程序在数据采集前仅包含一个上样步骤。然后，该阵列用于同时测定猪肉中的4种吩噻嗪类药物和5种苯二氮䓬类药物。这9种药物的检测限在0.001-0.01 ng/mL范围内，加标空白猪肉的回收率在63.5%-94.1%范围内。此外，该阵列可重复使用8次。一些实际猪肉样品的检测结果与超高效液相色谱法一致。
