Taghizadehghalehjoughi Ali, Sezen Selma, Hacimuftuoglu Ahmet, Güllüce Medine
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Erzurum, Turkey.
Department of Medical Pharmacology, Atatürk Univeristy, Erzurum, Turkey.
Mol Biol Rep. 2019 Apr;46(2):2523-2528. doi: 10.1007/s11033-019-04706-w. Epub 2019 Mar 22.
In this study, it was aimed to determine the effects of Amlodipine, a calcium channel blocker and vincristine (VCR) an antineoplastic, on human neuroblastomas using different doses. The cytotoxicity assays of the study were performed using the MTT method depending on time and concentration. After obtaining the mixture (up to 85% for SH-SY5Y) and sufficient branches (cortex neurons), the cells were treated with amlodipine (10 µM) and vincristine (0.5, 1 and 2 µg) at different concentrations for 24 h. MTT assay was performed by the commercially available kit (Sigma Aldrich, USA). Cells were harvested, washed and stained with PI and Annexin V, respectively, according to the manufacturer's protocol (Biovision, USA). Than analyzes were carried out. The results were quite impressive. When amlodipine (10 µM) was administered alone there was little change compared to the control. However, all doses of amlodipine (10 µM) and vincristine (0.5, 1 and 2 µg) were greater than the deaths in the doses alone (0.5, 1 and 2 µg) of vincristine alone. (P < 0.05). As a result, the combination of vincristine and amlodipine is more effective than vincristine alone in reducing the viability of cancer cells.
在本研究中，旨在确定钙通道阻滞剂氨氯地平和抗肿瘤药长春新碱（VCR）使用不同剂量对人神经母细胞瘤的影响。该研究的细胞毒性试验根据时间和浓度采用MTT法进行。在获得混合物（SH-SY5Y细胞达85%）和足够的分支（皮质神经元）后，将细胞用不同浓度的氨氯地平（10 μM）和长春新碱（0.5、1和2 μg）处理24小时。MTT试验通过市售试剂盒（美国西格玛奥德里奇公司）进行。根据制造商的方案（美国BioVision公司），分别收获细胞、洗涤并用PI和膜联蛋白V染色。然后进行分析。结果相当令人印象深刻。单独给予氨氯地平（10 μM）时，与对照组相比变化不大。然而，氨氯地平（10 μM）和长春新碱（0.5、1和2 μg）的所有剂量导致的死亡人数均高于单独使用长春新碱（0.5、1和2 μg）的剂量（P < 0.05）。结果表明，长春新碱和氨氯地平联合使用在降低癌细胞活力方面比单独使用长春新碱更有效。
