Nutrition and Clinical Services Division, International Centre for Diarrhoeal Disease Research Bangladesh (icddr,b), Dhaka, Bangladesh.
Department of Public Health, School of Medicine, Griffith University, Southport, Queensland, Australia.
Matern Child Nutr. 2019 Jul;15(3):e12815. doi: 10.1111/mcn.12815. Epub 2019 Apr 26.
Stunting in children is a global public health concern. We investigated how global DNA methylation relates to food intakes, dietary diversity, and development of stunting among 324 children aged 24-36 months in a slum community in Dhaka, Bangladesh. Stunted children (height-for-age z score ˂-2; n = 162) and their age- and sex-matched nonstunted counterparts (height-for-age z score ˃-1; n = 162) were selected by active community surveillance. We studied global DNA methylation, measured as 5-mC% content in whole blood. Dietary intake, anthropometric measurement, and sociodemographic information were obtained. In the multiple linear regression model, increased global DNA methylation level in children was significantly associated with consumption of lower amount of energy, coef: .034 (95% CI [.014, .053]); P = .001, protein, coef: .038 (95% CI [.019, .057]); P = .000, carbohydrate, coef: .027 (95% CI [.008, .047]); P = .006, zinc, coef: .020 (95% CI [.001, .039]); P = .043, total dietary intakes, coef: .020 (95% CI [.001, .039]); P = .043, and intake from plant sources, coef: .028 (95% CI [.009, .047]); P = .005, after adjusting for other covariates. Moreover, higher fruits and vegetables consumption was significantly associated with lower 5-mC% level, coef: -.022 (95% CI [-.041, -.002]); P = .028. Our findings suggest a significant association between low dietary intakes and increased global DNA methylation. We also found increased global DNA methylation in stunted children. To establish the relationship among the macronutrient intakes, global DNA methylation, and stunting, future prospective studies are warranted in resource-poor settings.
儿童发育迟缓是全球公共卫生关注的问题。我们研究了全血中 5- 甲基胞嘧啶（5-mC）%含量的全球 DNA 甲基化与孟加拉国达卡贫民窟社区 324 名 24-36 月龄儿童的饮食摄入、饮食多样性和发育迟缓发展之间的关系。通过主动社区监测，选择发育迟缓儿童（身高年龄 z 评分＜-2；n=162）和年龄、性别匹配的非发育迟缓儿童（身高年龄 z 评分＞-1；n=162）。我们研究了全球 DNA 甲基化，以全血中的 5-mC%含量来衡量。获得了饮食摄入、人体测量和社会人口学信息。在多元线性回归模型中，儿童体内的全球 DNA 甲基化水平升高与能量摄入减少显著相关，系数为：.034（95%CI [.014，.053]）；P=0.001，蛋白质，系数：.038（95%CI [.019，.057]）；P=0.000，碳水化合物，系数：.027（95%CI [.008，.047]）；P=0.006，锌，系数：.020（95%CI [.001，.039]）；P=0.043，总膳食摄入量，系数：.020（95%CI [.001，.039]）；P=0.043，以及植物来源的摄入量，系数：.028（95%CI [.009，.047]）；P=0.005，在调整其他协变量后。此外，较高的水果和蔬菜摄入量与较低的 5-mC%水平显著相关，系数为：-.022（95%CI [-.041，-.002]）；P=0.028。我们的研究结果表明，饮食摄入低与全球 DNA 甲基化水平升高之间存在显著关联。我们还发现发育迟缓儿童的全球 DNA 甲基化水平升高。为了确定宏量营养素摄入、全球 DNA 甲基化和发育迟缓之间的关系，在资源匮乏的环境中需要进行未来的前瞻性研究。
