Department of Clinical Biochemistry, Hematology and Immunology, Na Homolce Hospital, Prague 5, Czech Republic.
Physiol Res. 2019 Jun 30;68(3):385-394. doi: 10.33549/physiolres.933905. Epub 2019 Mar 22.
Abdominal aortic aneurysm (AAA) is a serious condition of unclear pathogenesis and progression. Two samples were collected from 48 patients during AAA surgery. One sample was collected from the aneurysm, the other from the aneurysm proximal neck where the tissue did not exhibit any aneurysmal changes. Subsequently, gene expression profiles using microarrays (Illumina) were compared in RNA extracted from the samples. Overall, 2,185 genes were found to be upregulated and 2,100 downregulated; from which 158 genes had a different expression with FDR<0.05 (False Discovery Rate) and FC>/=2 (Fold Change). Of this number, 115 genes were over-expressed and 43 under-expressed. The analysis of the gene list based on their biological pathways revealed that the regulation of inflammation was mediated by chemokine and cytokine signaling pathways, the integrin signaling pathway, and T and B cell activation. Moreover, a change was identified in the expression of genes involved in both intercellular and intracellular signaling systems.
腹主动脉瘤（AAA）是一种发病机制和进展尚不清楚的严重疾病。在 AAA 手术期间从 48 名患者中收集了两个样本。一个样本取自动脉瘤，另一个取自动脉瘤近端颈部，该组织没有任何动脉瘤样变化。随后，使用微阵列（Illumina）比较了从样本中提取的 RNA 的基因表达谱。总体而言，发现有 2185 个基因上调和 2100 个下调；其中 158 个基因的表达差异具有 FDR<0.05（假发现率）和 FC>/=2（倍数变化）。在这一数字中，有 115 个基因过表达，43 个基因低表达。根据其生物学途径对基因列表进行的分析表明，炎症的调节是通过趋化因子和细胞因子信号通路、整合素信号通路以及 T 和 B 细胞激活来介导的。此外，还发现了细胞间和细胞内信号系统中参与基因表达的变化。
