溴化乙锭对枯草芽孢杆菌转化的抑制作用。
Inhibition of transformation of Bacillus subtilis by ethidium bromide.
作者信息
Lopez P, Perez Ureña M T, Ballester S, Espinosa M
出版信息
Microbios. 1986;45(184-185):139-52.
PMID:3090402
Abstract
Incubation of donor DNA with ethidium bromide before addition to competent cells, strongly inhibited chromosomal transformation, plasmid transfer and phage-SPP1 DNA mediated transfection. There was no effect of the dye on the binding and entry of DNA into the cells. Analysis of the fate of transforming DNA treated with ethidium bromide showed a reduction in the amount of donor DNA associated with the recipient chromosome. The formation of this donor-recipient complex seems to be slowed down by the dye.
摘要
在将供体DNA添加到感受态细胞之前，先用溴化乙锭孵育，会强烈抑制染色体转化、质粒转移以及噬菌体-SPP1 DNA介导的转染。该染料对DNA与细胞的结合及进入细胞没有影响。对经溴化乙锭处理的转化DNA的命运分析表明，与受体染色体相关的供体DNA量减少。染料似乎减缓了这种供体-受体复合物的形成。
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