镁对枯草芽孢杆菌转染和转化的影响。

The effect of magnesium of transfection and transformation in Bacillus subtilis.

作者信息

López P, Pérez Ureña M T, Espinosa M

出版信息

Can J Microbiol. 1981 Oct;27(10):991-7. doi: 10.1139/m81-156.

Abstract

The influence of Mg2+ on phage SPP1 DNA-mediated transfection as compared with chromosomal transformation was studied. A differential influence of this cation in both processes was detectable by analyzing the competence development and the kinetics of appearance of transformants and transfectants. Binding and uptake of DNA and release of acid-soluble products by competent cells in high- and low-Mg2+ media was measured for 3H-labelled SPP1 and 3H-labelled Bacillus subtilis DNAs. Phage SPP1 DNA was shown to be subjected to endonucleolytic cleavage after exposure to competent cells.

摘要

研究了Mg2+对噬菌体SPP1 DNA介导的转染的影响，并与染色体转化进行了比较。通过分析感受态发育以及转化子和转染子出现的动力学，可检测到该阳离子在这两个过程中的差异影响。在高镁和低镁培养基中，测量了感受态细胞对3H标记的SPP1 DNA和3H标记的枯草芽孢杆菌DNA的DNA结合与摄取以及酸溶性产物的释放。结果表明，噬菌体SPP1 DNA在与感受态细胞接触后会发生内切核酸酶切割。