Krekic Szilvia, Nagy Dávid, Taneva Stefka G, Fábián László, Zimányi László, Dér András
Institute of Biophysics, Biological Research Centre, Hungarian Academy of Sciences, Temesvári krt. 62, P.O. Box 521, Szeged, 6701, Hungary.
Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., bl. 21, Sofia, 1113, Bulgaria.
Eur Biophys J. 2019 Jul;48(5):465-473. doi: 10.1007/s00249-019-01353-8. Epub 2019 Mar 23.
In this paper, the photocycle of the dried photoactive yellow protein film has been investigated in different humidity environments, in order to characterize its nonlinear optical properties for possible integrated optical applications. The light-induced spectral changes of the protein films were monitored by an optical multichannel analyser set-up, while the accompanying refractive index changes were measured with the optical waveguide lightmode spectroscopy method. To determine the number and kinetics of spectral intermediates in the photocycle, the absorption kinetic data were analysed by singular value decomposition and multiexponential fitting methods, whose results were used in a subsequent step of fitting a photocycle model to the data. The absorption signals of the films were found to be in strong correlation with the measured light-induced refractive index changes, whose size and kinetics imply that photoactive yellow protein may be a good alternative for utilization as an active nonlinear optical material in future integrated optical applications.
在本文中，已对干燥的光活性黄色蛋白膜在不同湿度环境下的光循环进行了研究，以表征其非线性光学特性，用于可能的集成光学应用。蛋白膜的光致光谱变化通过光学多通道分析仪装置进行监测，同时伴随的折射率变化则采用光波导光模光谱法进行测量。为了确定光循环中光谱中间体的数量和动力学，通过奇异值分解和多指数拟合方法对吸收动力学数据进行了分析，其结果在随后将光循环模型拟合到数据的步骤中得到应用。发现膜的吸收信号与测量的光致折射率变化密切相关，其大小和动力学表明光活性黄色蛋白可能是未来集成光学应用中用作活性非线性光学材料的良好替代品。
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