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生物功能化多孔硅微腔的微秒级全光调制

Microsecond All-Optical Modulation by Biofunctionalized Porous Silicon Microcavity.

作者信息

Petrovszki Dániel, Valkai Sándor, Kelemen Lóránd, Nagy László, Agarwal Vivechana, Krekic Szilvia, Zimányi László, Dér András

机构信息

Institute of Biophysics, Biological Research Centre, Eötvös Loránd Research Network, 6726 Szeged, Hungary.

Doctoral School of Multidisciplinary Medical Science, University of Szeged, 6720 Szeged, Hungary.

出版信息

Nanomaterials (Basel). 2023 Jul 14;13(14):2070. doi: 10.3390/nano13142070.

DOI:10.3390/nano13142070
PMID:37513080
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10385878/
Abstract

We successfully created a composite photonic structure out of porous silicon (PSi) microcavities doped by the photochromic protein, photoactive yellow protein (PYP). Massive incorporation of the protein molecules into the pores was substantiated by a 30 nm shift of the resonance dip upon functionalization, and light-induced reflectance changes of the device due to the protein photocycle were recorded. Model calculations for the photonic properties of the device were consistent with earlier results on the nonlinear optical properties of the protein, whose degree of incorporation into the PSi structure was also estimated. The successful proof-of-concept results are discussed in light of possible practical applications in the future.

摘要

我们成功地利用光致变色蛋白——光活性黄色蛋白(PYP)掺杂的多孔硅(PSi)微腔创建了一种复合光子结构。功能化后共振峰位发生30纳米的偏移,证实了蛋白质分子大量掺入到孔隙中,并且记录了由于蛋白质光循环导致的器件光诱导反射率变化。该器件光子特性的模型计算结果与该蛋白质非线性光学特性的早期研究结果一致,同时还估算了其掺入PSi结构的程度。结合未来可能的实际应用对这一成功的概念验证结果进行了讨论。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce3e/10385878/981022cf9832/nanomaterials-13-02070-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce3e/10385878/1e841601c4f9/nanomaterials-13-02070-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce3e/10385878/20c54fe23aa3/nanomaterials-13-02070-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce3e/10385878/2744997ad599/nanomaterials-13-02070-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce3e/10385878/22a527306d41/nanomaterials-13-02070-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce3e/10385878/cdc5966dd6d5/nanomaterials-13-02070-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce3e/10385878/981022cf9832/nanomaterials-13-02070-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce3e/10385878/1e841601c4f9/nanomaterials-13-02070-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce3e/10385878/20c54fe23aa3/nanomaterials-13-02070-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce3e/10385878/2744997ad599/nanomaterials-13-02070-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce3e/10385878/22a527306d41/nanomaterials-13-02070-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce3e/10385878/cdc5966dd6d5/nanomaterials-13-02070-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce3e/10385878/981022cf9832/nanomaterials-13-02070-g006.jpg

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All-Optical Switching Demonstrated with Photoactive Yellow Protein Films.用光活性黄色蛋白膜实现全光交换。
Biosensors (Basel). 2021 Oct 31;11(11):432. doi: 10.3390/bios11110432.
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Nonlinear Optical Investigation of Microbial Chromoproteins.微生物色蛋白的非线性光学研究
Front Plant Sci. 2020 Oct 21;11:547818. doi: 10.3389/fpls.2020.547818. eCollection 2020.
4
Spectrokinetic characterization of photoactive yellow protein films for integrated optical applications.用于集成光学应用的光活性黄色蛋白薄膜的光谱动力学表征。
Eur Biophys J. 2019 Jul;48(5):465-473. doi: 10.1007/s00249-019-01353-8. Epub 2019 Mar 23.
5
Porous silicon/photosynthetic reaction center hybrid nanostructure.多孔硅/光合作用反应中心杂化纳米结构。
Langmuir. 2012 Aug 14;28(32):11866-73. doi: 10.1021/la301888p. Epub 2012 Jul 31.
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Light-harvesting bio-nanomaterial using porous silicon and photosynthetic reaction center.利用多孔硅和光合反应中心的光捕获生物纳米材料。
Nanoscale Res Lett. 2012 Jul 17;7(1):400. doi: 10.1186/1556-276X-7-400.
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Protein-based ultrafast photonic switching.基于蛋白质的超快光子开关。
Opt Express. 2011 Sep 26;19(20):18861-70. doi: 10.1364/OE.19.018861.
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Biosensing and protein fluorescence enhancement by functionalized porous silicon devices.功能化多孔硅器件的生物传感与蛋白质荧光增强
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Characterization of the solution structure of the M intermediate of photoactive yellow protein using high-angle solution x-ray scattering.利用高角度溶液X射线散射对光活性黄色蛋白M中间体的溶液结构进行表征。
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