Sundararaj Naveen, Kalagatur Naveen Kumar, Mudili Venkataramana, Krishna Kadirvelu, Antonysamy Michael
1Department of Microbiology, PSG College of Arts and Science, Coimbatore, Tamilnadu 641020 India.
Toxicology and Immunology Division, DRDO-BU-Center for Life Sciences, Coimbatore, Tamilnadu 641020 India.
J Food Sci Technol. 2019 Feb;56(2):1016-1026. doi: 10.1007/s13197-019-03568-1. Epub 2019 Jan 21.
is one of the major food contaminants worldwide, and its enterotoxins are documented as food poisoning and bioterrorism agents. In the present study, an attempt was made to account on the incidences of toxigenic and its antibiotic resistance profiles in ready to eat bakery food products from different parts of Southern India (Andhra Pradesh, Karnataka, Kerala, Tamil Nadu, and Telangana). A total of 100 food samples, including milk, cake, cheese and chicken products were assessed for and Staphylococcal Enterotoxin B (SEB) by PCR. Among the subjected food samples, a total of 51 isolates belong to genus and out of that, 34 isolates were . Among 34 isolates, 14 isolates were found positive for SEB. The PCR results were further co-evaluated with in-house developed aptamer linked immunosorbent assay (ALISA) for the specific and sensitive detection of SEB. The obtained ALISA results were promising and found consistent with PCR analysis. Furthermore, 24%, 47%, 91%, 82%, 59%, and 47% of isolates were found resistant to chloramphenicol, methicillin, penicillin, ampicillin, erythromycin, and oxacillin, respectively and concluded as a multidrug resistance (MDR). In conclusion, the present study revealed high presence of toxigenic and MDR resistant species among the studied regions of Southern India. The present study cautions the need of stringent food safety regulations in India to control the toxigenic and MDR from food sources and to minimize the risks associated with .
是全球主要的食品污染物之一，其肠毒素被记录为食物中毒和生物恐怖主义制剂。在本研究中，试图统计印度南部不同地区（安得拉邦、卡纳塔克邦、喀拉拉邦、泰米尔纳德邦和特伦甘纳邦）即食烘焙食品中产毒素的发生率及其抗生素耐药性概况。通过聚合酶链反应（PCR）对总共100份食品样本（包括牛奶、蛋糕、奶酪和鸡肉产品）进行了 和葡萄球菌肠毒素B（SEB）的评估。在受试食品样本中，共有51株分离株属于 属，其中34株为 。在34株 分离株中，有14株SEB检测呈阳性。PCR结果进一步与内部开发的适体连接免疫吸附测定（ALISA）共同评估，用于SEB的特异性和灵敏检测。获得的ALISA结果很有前景，并且与PCR分析结果一致。此外，分别有24%、47%、91%、82%、59%和47%的 分离株对氯霉素、甲氧西林、青霉素、氨苄青霉素、红霉素和苯唑西林耐药，并被判定为多重耐药（MDR）。总之，本研究揭示了印度南部研究区域中产毒素和多重耐药的 菌种的高存在率。本研究提醒印度需要严格的食品安全法规，以控制食品来源中产毒素和多重耐药的 ，并将与之相关的风险降至最低。
