Shin Ho Sik, Lee Seung-Keun, Kim Saeromi, Kim Hyung-Jun, Chae Won Seok, Park Sun Ah
Department of Neurology, Soonchunhyang University Bucheon Hospital, Bucheon, Korea.
Department of Anesthesiology, Soonchunhyang University Bucheon Hospital, Bucheon, Korea.
Dement Neurocogn Disord. 2016 Dec;15(4):122-128. doi: 10.12779/dnd.2016.15.4.122. Epub 2016 Dec 31.
Alzheimer's disease (AD) is characterized by the accumulation of amyloid-β proteins (Aβ). In this study we explored the correlation of plasma Aβ40 and Aβ42 concentrations with Aβ42, total tau (tTau) and phosphorylated tau 181 (pTau181) levels in cerebrospinal fluid (CSF) in AD and control subjects to further understand the characteristics of plasma Aβ proteins levels.
The consecutive subjects (44 AD and 47 controls) in this study were recruited. The plasma levels of Aβ40 and Aβ42 were measured using a commercially available sandwich enzyme-linked immunosorbent assay (ELISA) kits. And the corresponding CSFs were analyzed in terms of Aβ42, tTau and pTau181 concentrations using INNOTEST ELISA kits. Further, the albumin levels were measured both in serum and CSF and albumin ratio was obtained to check the integrity of blood-brain barrier.
CSF Aβ42 concentrations were significantly decreased while tTau and pTau181 levels were significantly increased in AD subjects. The plasma levels of Aβ42 were significantly lower (=0.007), while the Aβ40/Aβ42 ratio was significantly higher (<0.001) in AD patients than in controls. The overall plasma Aβ42 levels showed a positive correlation with those of CSF Aβ42 (=0.001), but not with the others in CSF. In subgroup analysis, the CSF Aβ42 demonstrated positive correlation with not only plasma Aβ42 but also Aβ40 levels in controls. However, no significant relationship was noted between plasma and CSF Aβ proteins in AD group.
The plasma Aβ42 and Aβ40 concentrations were shown to have a close relationship with CSF Aβ42 levels in controls, but not in AD subjects. Our results suggest that the correlation between plasma Aβ40 and CSF Aβ42 levels is perturbed in AD.
阿尔茨海默病（AD）的特征是β淀粉样蛋白（Aβ）的积累。在本研究中，我们探讨了AD患者和对照者血浆Aβ40和Aβ42浓度与脑脊液（CSF）中Aβ42、总tau蛋白（tTau）和磷酸化tau181（pTau181）水平的相关性，以进一步了解血浆Aβ蛋白水平的特征。
本研究纳入了连续的受试者（44例AD患者和47例对照者）。使用市售的夹心酶联免疫吸附测定（ELISA）试剂盒测量血浆Aβ40和Aβ42水平。并使用INNOTEST ELISA试剂盒分析相应脑脊液中Aβ42、tTau和pTau181的浓度。此外，测量血清和脑脊液中的白蛋白水平，并计算白蛋白比值以检查血脑屏障的完整性。
AD患者脑脊液中Aβ42浓度显著降低，而tTau和pTau181水平显著升高。AD患者的血浆Aβ42水平显著降低（=0.007），而Aβ40/Aβ42比值显著升高（<0.001）。血浆Aβ42总体水平与脑脊液Aβ42水平呈正相关（=0.001），但与脑脊液中的其他指标无相关性。在亚组分析中，对照组脑脊液Aβ42不仅与血浆Aβ42呈正相关，还与Aβ40水平呈正相关。然而，AD组血浆和脑脊液Aβ蛋白之间未发现显著关系。
血浆Aβ42和Aβ40浓度在对照组中与脑脊液Aβ42水平密切相关，但在AD患者中并非如此。我们的结果表明，AD患者血浆Aβ40和脑脊液Aβ42水平之间的相关性受到干扰。
