Wollweber L, Wisniewski H G
Acta Histochem Suppl. 1986;33:305-7.
A modification of a method is described for detection of antigens and antibodies by means of nitrocellulose paper discs as high-capacity solid phase antigen carrier and mouse PAP. Mouse IgG spotted on nitrocellulose paper may be easily detected at a level of 1 ng. Detection of specific antibodies is possible down to a concentration of 80 ng per ml. This method is of practical importance for the screening of hybridoma supernatants.
本文描述了一种方法的改进，该方法利用硝酸纤维素纸圆盘作为高容量固相抗原载体和小鼠过氧化物酶抗过氧化物酶复合物（PAP）来检测抗原和抗体。点样在硝酸纤维素纸上的小鼠IgG在1 ng水平时即可轻松检测到。特异性抗体的检测下限可达每毫升80 ng。该方法对于杂交瘤细胞培养上清液的筛选具有实际重要性。
