Antigen spot test for the detection of specific antibodies in the nanogram range by means of the PAP-technique.

A modification of a method is described for detection of antigens and antibodies by means of nitrocellulose paper discs as high-capacity solid phase antigen carrier and mouse PAP. Mouse IgG spotted on nitrocellulose paper may be easily detected at a level of 1 ng. Detection of specific antibodies is possible down to a concentration of 80 ng per ml. This method is of practical importance for the screening of hybridoma supernatants.