Surerus Campos D G, Queiroz da Cruz M, Rodrigues R M, Tendler M, Katz N, Oliveira Lima A
Mem Inst Oswaldo Cruz. 1986 Apr-Jun;81(2):135-44.
The main steps of a sensitive and quantitative technique for the simultaneous determination, in the same microplate, of antigen, antibody and specific immune complexes has been described. The assay involved principles of the double sandwich technique used in the "Enzyme-Linked Immunosorbent Assay" (ELISA). The technique proved to be useful for the estimation of antigen, antibody and specific immune complexes in sera of patients with human chronic hepato-intestinal schistosomiasis.
本文描述了一种灵敏且定量的技术的主要步骤,该技术可在同一微孔板中同时测定抗原、抗体和特异性免疫复合物。该检测方法涉及“酶联免疫吸附测定法”(ELISA)中使用的双夹心技术原理。该技术被证明可用于评估人类慢性肝肠血吸虫病患者血清中的抗原、抗体和特异性免疫复合物。