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光反应在视杆细胞外段的传播:来自膜片钳记录的估计

Spread of the light response along the rod outer segment: an estimate from patch-clamp recordings.

作者信息

Matthews G

出版信息

Vision Res. 1986;26(4):535-41. doi: 10.1016/0042-6989(86)90002-7.

DOI:10.1016/0042-6989(86)90002-7
PMID:3090779
Abstract

Longitudinal spread of the light response was studied using cell-attached patch-clamp recordings from the outer segments of isolated rod photoreceptors from toad retina. The effective photon-collecting area over which photoisomerizations influenced the membrane current through the recorded patch of membrane was determined by measuring the probability of a detectable response as a function of flash photon density (i.e. a "frequency-of-seeing" experiment). The effective collecting area averaged 0.40 micron2, which corresponds to a total sampling length of approximately 1 micron for the patch electrode.

摘要

利用从蟾蜍视网膜分离出的视杆光感受器外段进行细胞贴附式膜片钳记录,研究了光反应的纵向传播。通过测量可检测反应的概率作为闪光光子密度的函数(即“看见频率”实验),确定了光异构化通过记录的膜片影响膜电流的有效光子收集面积。有效收集面积平均为0.40平方微米,这对应于膜片电极的总采样长度约为1微米。

相似文献

1
Spread of the light response along the rod outer segment: an estimate from patch-clamp recordings.光反应在视杆细胞外段的传播:来自膜片钳记录的估计
Vision Res. 1986;26(4):535-41. doi: 10.1016/0042-6989(86)90002-7.
2
Spatial spread of activation and background desensitization in toad rod outer segments.蟾蜍视杆细胞外段激活的空间扩散及背景脱敏
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Two components of electrical dark noise in toad retinal rod outer segments.蟾蜍视网膜视杆细胞外段电暗噪声的两个组成部分。
J Physiol. 1980 Dec;309:591-621. doi: 10.1113/jphysiol.1980.sp013529.
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Dependence of the single photon response on longitudinal position of absorption in toad rod outer segments.蟾蜍视杆细胞外段中单光子响应与吸收纵向位置的相关性。
J Physiol. 1983 Oct;343:147-59. doi: 10.1113/jphysiol.1983.sp014886.
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Properties of ion channels closed by light and opened by guanosine 3',5'-cyclic monophosphate in toad retinal rods.蟾蜍视网膜视杆细胞中被光关闭并被鸟苷 3',5'-环磷酸打开的离子通道特性
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Dark noise in the outer segment membrane current of green rod photoreceptors from toad retina.蟾蜍视网膜绿色视杆光感受器外段膜电流中的暗噪声。
J Physiol. 1984 Apr;349:607-18. doi: 10.1113/jphysiol.1984.sp015176.
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Effects of calcium and guanosine-3',5'-cyclic-monophosphoric acid on receptor potentials of toad rods.钙和鸟苷-3',5'-环一磷酸对蟾蜍视杆细胞感受器电位的影响。
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Physiological characteristics of single green rod photoreceptors from toad retina.蟾蜍视网膜单个绿色视杆光感受器的生理特征
J Physiol. 1983 Sep;342:347-59. doi: 10.1113/jphysiol.1983.sp014855.
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The relation between excitation and adaptation within the rod's outer segment.视杆细胞外段内兴奋与适应之间的关系。
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Guanosine 3',5'-cyclic monophosphate-activated conductance studied in a truncated rod outer segment of the toad.在蟾蜍截短的视杆外段中研究的3',5'-环磷酸鸟苷激活的电导。
J Physiol. 1988 Jan;395:731-53. doi: 10.1113/jphysiol.1988.sp016943.

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J Physiol. 1999 Sep 15;519 Pt 3(Pt 3):679-92. doi: 10.1111/j.1469-7793.1999.0679n.x.
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Guanosine 3',5'-cyclic monophosphate-activated conductance studied in a truncated rod outer segment of the toad.在蟾蜍截短的视杆外段中研究的3',5'-环磷酸鸟苷激活的电导。
J Physiol. 1988 Jan;395:731-53. doi: 10.1113/jphysiol.1988.sp016943.
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Properties of ion channels closed by light and opened by guanosine 3',5'-cyclic monophosphate in toad retinal rods.蟾蜍视网膜视杆细胞中被光关闭并被鸟苷 3',5'-环磷酸打开的离子通道特性
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Single-channel recordings demonstrate that cGMP opens the light-sensitive ion channel of the rod photoreceptor.单通道记录表明,环磷酸鸟苷(cGMP)打开了视杆光感受器的光敏离子通道。
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Some conjectures on the design of a rod outer segment.关于杆状外节设计的一些猜想。
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