B族血清群链球菌中的β-D-半乳糖苷酶活性

beta-D-galactosidase activity in streptococci of serological group B.

作者信息

Lämmler C, Schaufuss P, Blobel H

出版信息

Zentralbl Bakteriol Mikrobiol Hyg A. 1986 Apr;261(2):167-9. doi: 10.1016/s0176-6724(86)80032-3.

Abstract

Group B streptococci isolated from humans differed significantly in beta-D-galactosidase-activity from those of bovine mastitis. This could be demonstrated in a relatively simple and rapid test using a fluorogenic 4-methylumbelliferyl-beta-D-galactoside conjugate. Only 10 (12%) of 82 group B streptococcal cultures from human produced beta-D-galactosidase. On the other hand, 74 (96%) of 77 "bovine" cultures formed this enzyme. Thus, beta-D-galactosidase activity could be used as an additional marker for the differentiation between group B streptococci of human and bovine origin.

摘要

从人类分离出的B族链球菌在β-D-半乳糖苷酶活性方面与牛乳腺炎的B族链球菌有显著差异。这可以通过使用荧光4-甲基伞形酮基-β-D-半乳糖苷共轭物的相对简单快速的试验来证明。82株来自人类的B族链球菌培养物中只有10株（12%）产生β-D-半乳糖苷酶。另一方面，77株“牛源”培养物中有74株（96%）形成了这种酶。因此，β-D-半乳糖苷酶活性可作为区分人类和牛源B族链球菌的一个额外标志物。