Watts J L, Salmon S A, Yancey R J
Animal Health Therapeutics Research, Upjohn Company, Kalamazoo, MI 49001.
J Dairy Sci. 1993 Jun;76(6):1740-3. doi: 10.3168/jds.S0022-0302(93)77506-2.
The medium of Rambach was modified to permit differentiation of mastitis streptococci. A total of 377 streptococci isolated from bovine IMI was used in the study. Of the 159 strains identified as Streptococcus uberis, 151 strains (94.9%) were beta-galactosidase-positive and yielded blue colonies on modified Rambach agar. In comparison, Streptococcus agalactiae, Streptococcus dysgalactiae, Enterococcus saccharolyticus, or Enterococcus faecalis strains were negative for propylene glycol utilization (red colonies) or beta-galactosidase production; all strains yielded colorless colonies on modified Rambach agar. However, 5 of 35 (14.3%) Streptococcus equinus strains were also positive for beta-galactosidase. Results indicate that modified Rambach agar is a convenient medium for the differentiation of the Strep. uberis from the other mastitis streptococci. Furthermore, modified Rambach agar could be easily incorporated as a screening medium for Strep. uberis in mastitis bacteriology laboratories.
对兰巴赫培养基进行了改良，以实现乳腺炎链球菌的鉴别。本研究共使用了从牛亚临床型乳房炎中分离出的377株链球菌。在鉴定为乳房链球菌的159株菌株中，有151株（94.9%）β-半乳糖苷酶呈阳性，在改良的兰巴赫琼脂上产生蓝色菌落。相比之下，无乳链球菌、停乳链球菌、解糖肠球菌或粪肠球菌菌株对丙二醇利用呈阴性（红色菌落）或不产生β-半乳糖苷酶；所有菌株在改良的兰巴赫琼脂上均产生无色菌落。然而，35株马肠链球菌中有5株（14.3%）β-半乳糖苷酶也呈阳性。结果表明，改良的兰巴赫琼脂是一种方便的培养基，可用于从其他乳腺炎链球菌中鉴别出乳房链球菌。此外，改良的兰巴赫琼脂可轻松用作乳腺炎细菌学实验室中乳房链球菌的筛选培养基。
