Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecular Chemistry, College of Chemistry & Materials Science, Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry , Northwest University , Xi'an 710127 , China.
School of Chemistry and Chemical Engineering , Xi'an University of Architecture and Technology , Xi'an 710055 , China.
Anal Chem. 2019 Apr 16;91(8):5125-5132. doi: 10.1021/acs.analchem.8b05673. Epub 2019 Apr 3.
Label-free biosensing based on the nanoporous anodic alumina (NAA) membrane emerged as a versatile biosensing platform in the recent decade. In the present work, we developed a new immunosensing strategy based on the nanochannels of NAA and the ion pair interaction mediated by electrochemistry of C. The NAA served as the matrix for the immobilization of the capture antibodies. The incubation of target antigens resulted in the formation of the immunocomplexes and thus an increase of the steric hindrance of the nanochannels. Therefore, the concentration of the redox probe transported through the nanochannels decreases, which can be detected at the working electrode modified with C. Herein, we initially found that the cathodic peak ascribed to the reduction of C to C was obviously enhanced by the presence of the redox probe K[Fe(CN)] and which was contributed to the formation of a ternary ion association complex among C, tetraoctylammonium bromide, and K[Fe(CN)]. Therefore, the transportation of K[Fe(CN)] though the NAA-based bionanochannels can be detected by a C modified electrode with an amplified signal. Choosing human epididymis protein 4 (HE4) as the model target, a linear range of 1.0 ng mL to 100 ng mL can be established between the peak current obtained from the differential pulse voltammetric response of the platform and the concentration of HE4. The detection limit was 0.2 ng mL. This study not only provides a new avenue to develop the other nanochannel-based biosensing platform for a variety of other disease biomarkers but also contributes to the electrochemistry of fullerene.
基于纳米多孔阳极氧化铝（NAA）膜的无标记生物传感技术在最近十年中成为一种多功能生物传感平台。在本工作中，我们开发了一种基于 NAA 的纳米通道和电化学介导的离子对相互作用的新型免疫传感策略。NAA 用作固定捕获抗体的基质。目标抗原的孵育导致免疫复合物的形成，从而增加纳米通道的空间位阻。因此，通过纳米通道传输的氧化还原探针的浓度降低，这可以在修饰有 C 的工作电极上进行检测。在此，我们最初发现，存在氧化还原探针 K[Fe(CN)]时，归因于 C 还原为 C 的阴极峰明显增强，这归因于 C、四辛基溴化铵和 K[Fe(CN)]之间形成三元离子缔合复合物。因此，可以通过修饰有 C 的电极检测通过 NAA 基生物纳米通道的 K[Fe(CN)]的传输，信号得到放大。选择人附睾蛋白 4（HE4）作为模型靶标，可在平台的差分脉冲伏安响应获得的峰电流和 HE4 浓度之间建立 1.0ng mL 至 100ng mL 的线性范围。检测限为 0.2ng mL。这项研究不仅为开发其他基于纳米通道的生物传感平台提供了新途径，用于检测各种其他疾病生物标志物，而且为富勒烯的电化学提供了新途径。
