Department of Dermatology, Zealand University Hospital, Roskilde, Denmark.
Health Sciences Faculty, University of Copenhagen, Copenhagen, Denmark.
J Eur Acad Dermatol Venereol. 2019 Sep;33(9):1775-1780. doi: 10.1111/jdv.15597. Epub 2019 May 6.
Hidradenitis suppurativa (HS) is a chronic inflammatory skin disease defined by recurrent nodules, tunnels and scarring involving the intertriginous regions. Recent next-generation sequencing (NGS) studies suggest genera such as Prevotella spp., Peptoniphilus spp. and Porphyromonas spp. are associated with chronic and early HS lesions. However, a systematic investigation of the bacterial microbiome in HS tunnels remains unexplored using NGS.
We aimed to investigate the bacterial composition of the luminal white gelatinous material found in HS tunnels using NGS.
An exploratory study of patients with diagnosis of HS (n = 32) with tunnels. The tunnels were present either in the groin (n = 17) or in the axilla (n = 15). During deroofing of the tunnels, a sterile E-swab was taken of the luminal gelatinous material. The samples were investigated using NGS targeting 16S ribosomal RNA.
The skin microbiome was characterized in 32 HS patients. Overall, five microbiome types were identified: Porphyromonas spp. (type I), Corynebacterium spp., (type II), Staphylococcus spp. (type III), Prevotella spp. (type IV) and Acinetobacter spp (type V). Porphyromonas spp. (type I) and Prevotella spp. (IV) were the most frequent genera found the tunnels.
This study points to a potential association between the presence of certain anaerobic bacteria (Porphyromonas spp., Prevotella spp.) and HS tunnels. It may be speculated that these two genera are associated with the pathogenesis in HS either as drivers or as biomarkers.
化脓性汗腺炎（HS）是一种慢性炎症性皮肤病，其特征为反复发作的结节、瘘管和瘢痕，涉及皱褶部位。最近的下一代测序（NGS）研究表明，普雷沃菌属、消化链球菌属和卟啉单胞菌属等菌属与慢性和早期 HS 病变有关。然而，利用 NGS 对 HS 瘘管中的细菌微生物组进行系统研究仍有待探索。
我们旨在使用 NGS 研究 HS 瘘管中腔白色胶状物质的细菌组成。
对 32 例诊断为 HS（n=32）且存在瘘管的患者进行了一项探索性研究。这些瘘管位于腹股沟（n=17）或腋窝（n=15）。在切除瘘管的屋顶时，用无菌 E-swab 采集腔室胶状物质。使用针对 16S 核糖体 RNA 的 NGS 对样本进行了研究。
对 32 例 HS 患者的皮肤微生物组进行了特征描述。总体上，鉴定出了五种微生物组类型：卟啉单胞菌属（I 型）、棒状杆菌属（II 型）、葡萄球菌属（III 型）、普雷沃菌属（IV 型）和不动杆菌属（V 型）。在瘘管中最常发现的菌属是卟啉单胞菌属（I 型）和普雷沃菌属（IV 型）。
本研究表明某些厌氧菌（卟啉单胞菌属、普雷沃菌属）与 HS 瘘管之间存在潜在关联。可以推测这两个属与 HS 的发病机制有关，无论是作为驱动因素还是作为生物标志物。
