Meesters Arne A, Nieboer Marilin J, Almasian Mitra, Georgiou Giota, de Rie Menno A, Verdaasdonk Rudolf M, Wolkerstorfer Albert
Department of Dermatology, Amsterdam UMC, University of Amsterdam, Meibergdreef 9, NL-1105 AZ, Amsterdam, The Netherlands.
Department of Physics and Medical Technology, Amsterdam UMC, VU University, De Boelelaan 1117, NL-1081 HV, Amsterdam, The Netherlands.
Lasers Surg Med. 2019 Oct;51(8):709-719. doi: 10.1002/lsm.23088. Epub 2019 Mar 25.
Topical drug delivery can be increased by pretreatment of the skin with ablative fractional laser (AFXL). Several physical penetration enhancement techniques have been investigated to further improve AFXL-assisted drug delivery. This study investigated the influence of three of these techniques, namely massage, acoustic pressure wave treatment, and pressure vacuum alterations (PVP) on the distribution of the fluorescent drug indocyanine green (ICG) at different depths in the skin after topical application on AFXL pretreated skin.
In ex vivo human skin, test regions were pretreated with AFXL (10,600 nm, channel depth 300 μm, channel width 120 μm, density 15%). Subsequently, ICG was applied, followed by massage, acoustic pressure wave treatment or PVP. ICG fluorescence intensity (FI) was assessed after 1, 3, and 24 hours at several depths using fluorescence photography.
FI was higher when using enhancement techniques compared to control (AFXL-only) up to 3 hours application time (P < 0.05). After 3 hours, mean surface FI was highest after acoustic pressure wave treatment (61.5 arbitrary units; AU), followed by massage (57.5AU) and PVP (46.9AU), respectively (for comparison: AFXL-only 31.6AU, no pretreatment 14.9AU). Comparable or higher FI was achieved already after 1 hour with enhancement techniques compared to 3-24 hours application time without. After 24 hours, no significant differences between enhancement techniques and AFXL-only were observed (P = 0.31).
Penetration enhancement techniques, especially acoustic pressure wave treatment and massage, result in improved drug accumulation in AFXL-pretreated skin and reduce the application time needed. Lasers Surg. Med. © 2019 The Authors. Lasers in Surgery and Medicine Published by Wiley Periodicals, Inc.
通过剥脱性分数激光（AFXL）对皮肤进行预处理可增加局部药物递送。人们已经研究了几种物理渗透增强技术，以进一步改善AFXL辅助的药物递送。本研究调查了其中三种技术，即按摩、声压波治疗和压力真空改变（PVP）对荧光药物吲哚菁绿（ICG）在AFXL预处理皮肤局部应用后在皮肤不同深度分布的影响。
在离体人皮肤上，用AFXL（10600nm，通道深度300μm，通道宽度120μm，密度15%）对测试区域进行预处理。随后，应用ICG，接着进行按摩、声压波治疗或PVP。使用荧光摄影在1、3和24小时后在几个深度评估ICG荧光强度（FI）。
与对照组（仅AFXL）相比，在长达3小时的应用时间内，使用增强技术时FI更高（P<0.05）。3小时后，声压波治疗后的平均表面FI最高（61.5任意单位；AU），其次是按摩（57.5AU）和PVP（46.9AU）（相比之下：仅AFXL为31.6AU，未预处理为14.9AU）。与未使用增强技术的3 - 24小时应用时间相比，使用增强技术在1小时后即可达到相当或更高的FI。24小时后，增强技术与仅AFXL之间未观察到显著差异（P = 0.31）。
渗透增强技术，尤其是声压波治疗和按摩，可改善AFXL预处理皮肤中的药物蓄积，并减少所需的应用时间。《激光外科与医学》。©2019作者。《激光外科与医学》由威利期刊公司出版。
