Clin Hemorheol Microcirc. 2019;72(4):365-374. doi: 10.3233/CH-180536.
Topical negative pressure wound therapy (TNPWT) is one of the most frequently used techniques in wound treatment. But some of the underlying mechanisms still remain unclear. One possible explanation is an improved microcirculation by TNPWT.
This study investigated the influence of TNPWT on microcirculation on intact skin in real-time.
In healthy individuals, we performed a combined tissue - laser/photo - spectrometry technique to monitor changes of 4 different microcirculation parameters in real-time: The local blood flow, the capillary-venous oxygen saturation, the blood flow velocity and the relative amount of hemoglobin. We compared these parameters using two different protocols: a continuously (VAC ON 60/OFF 60) and discontinuously (VAC ON 30/OFF 60/ON 5) application.
Our results demonstrate a significant increase of all four measured parameters during the active TNPWT and the pressure free period. The comparison of two different protocols shows an advantage of the examined parameters using a discontinuous TNPWT application.
Our study demonstrated the changes of the microvascular tissue perfusion in intact human skin under the conditions of negative pressure and may thereby offer a broader understanding of mechanisms underlying the TNPWT.
负压伤口治疗（TNPWT）是伤口治疗中最常用的技术之一。但一些潜在的机制仍不清楚。一种可能的解释是 TNPWT 改善了微循环。
本研究实时研究 TNPWT 对完整皮肤微循环的影响。
在健康个体中，我们使用联合组织激光/光光谱技术实时监测 4 种不同微循环参数的变化：局部血流量、毛细血管静脉血氧饱和度、血流速度和血红蛋白相对量。我们使用两种不同的方案比较这些参数：连续（VAC ON 60/OFF 60）和不连续（VAC ON 30/OFF 60/ON 5）应用。
我们的结果表明，在主动 TNPWT 和无压期间，所有四个测量参数都显著增加。两种不同方案的比较表明，间断 TNPWT 应用对检查参数具有优势。
本研究在负压条件下证明了完整人体皮肤中小血管组织灌注的变化，从而为 TNPWT 的潜在机制提供了更广泛的理解。
