Fekkes D, Bruinvels J
Biol Psychiatry. 1986 Aug;21(10):951-9. doi: 10.1016/0006-3223(86)90269-6.
A disturbance of serine-glycine metabolism in a group of patients who became psychotic after oral intake of serine may be due to any serine-related enzyme abnormality. In order to elucidate this problem, we studied several enzymes in fibroblasts obtained from these patients. First, the enzyme serine hydroxymethyltransferase was investigated. The apparent Km values for serine, L-tetrahydrofolate (H4folate), and pyridoxal 5'-phosphate, as well as the maximal velocities of the forward and backward reactions measured in fibroblasts obtained from patients, were not different from those in the cells from controls. We also measured the activities of another three enzymes of the folic acid cycle, viz., 5,10-methylene-H4folate dehydrogenase, 10-formyl-H4folate synthetase, and 5,10-methenyl-H4folate cyclohydrolase, as well as the enzyme cystathionine beta-synthase. Again, no differences were found among these enzymes in fibroblasts from patients and controls. It can be concluded that the psychotic symptoms occurring after the administration of serine are not the result of any malfunctioning of the enzymes investigated.
一组在口服丝氨酸后出现精神症状的患者中，丝氨酸-甘氨酸代谢紊乱可能归因于任何与丝氨酸相关的酶异常。为了阐明这个问题，我们研究了从这些患者身上获取的成纤维细胞中的几种酶。首先，对丝氨酸羟甲基转移酶进行了研究。在从患者身上获取的成纤维细胞中测得的丝氨酸、L-四氢叶酸（H4叶酸）和磷酸吡哆醛的表观Km值，以及正向和反向反应的最大速度，与对照组细胞中的值没有差异。我们还测量了叶酸循环中的另外三种酶的活性，即5,10-亚甲基-H4叶酸脱氢酶、10-甲酰-H4叶酸合成酶和5,10-亚甲四氢叶酸环水解酶，以及胱硫醚β-合酶。同样，在患者和成纤维细胞中的这些酶与对照组之间未发现差异。可以得出结论，服用丝氨酸后出现的精神症状不是所研究酶功能异常的结果。
