Danafar Hossein, Asadi Farnaz, Sharafi Ali, Manjili Hamidreza Kheiri
Department of Pharmaceutical Biomaterials, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
Department of Biology, Faculty of Genetics, East Tehran Branch, Islamic Azad University, Tehran, Iran.
Drug Res (Stuttg). 2019 Sep;69(9):496-504. doi: 10.1055/a-0855-8464. Epub 2019 Mar 25.
Chitosan-coated magnetic nanoparticles are an appropriate drug delivery method which can improve the therapeutic properties of chemotherapy agents and also can be useful as MRI contrast agent for early cancer diagnosis. This research discovers the optimization of the possible therapeutic effects of Chitosan- citric acid- FeO- CUR quartets. Chitosan as a natural polymer can use to encapsulate citric acid modified FeO nanoparticles. The successful preparation of CUR-loaded nano-carriers was confirmed by X-ray diffraction (XRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), vibrating sample magnetometer (VSM) and transmission electron microscopy (TEM) techniques. Moreover, the hemolysis test was used for the study of hemobiocompatibility. The loading capacity and encapsulation efficiency of CUR molecules were 11±0.09% and 49.5±0.41%, respectively. The anticancer effect of the void of CUR and CUR-loaded nano-carriers were compared by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay on the treated MCF-7 cell lines. It can be concluded that the use of these nanoparticles are a better and more efficient approach for the controlled and slow release of CUR in the cancer treatment.
壳聚糖包覆的磁性纳米颗粒是一种合适的药物递送方法，它可以改善化疗药物的治疗特性，并且还可用作磁共振成像（MRI）造影剂用于早期癌症诊断。本研究探索了壳聚糖 - 柠檬酸 - 氧化亚铁 - 姜黄素（CUR）四联体可能的治疗效果的优化。壳聚糖作为一种天然聚合物，可用于包封柠檬酸修饰的氧化亚铁纳米颗粒。通过X射线衍射（XRD）、热重分析（TGA）、差示扫描量热法（DSC）、傅里叶变换红外光谱（FTIR）、振动样品磁强计（VSM）和透射电子显微镜（TEM）技术证实了负载姜黄素的纳米载体的成功制备。此外，溶血试验用于研究血液生物相容性。姜黄素分子的负载量和包封率分别为11±0.09%和49.5±0.41%。通过3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基四氮唑溴盐试验比较了空白姜黄素和负载姜黄素的纳米载体对经处理的MCF - 7细胞系的抗癌作用。可以得出结论，在癌症治疗中使用这些纳米颗粒是一种更好、更有效的姜黄素控释和缓释方法。
