Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk, KSA
Department of Microbiology and Immunology, Faculty of Medical Laboratory Sciences, Alzaiem Alazhari University, 71491, Tabuk, KSA
Infect Disord Drug Targets. 2020;20(4):491-494. doi: 10.2174/1871526519666190326141550.
Heteroresistant Mycobacterium tuberculosis (MTB) is defined as a group of drug-susceptible and resistant bacteria in a single clinical specimen from tuberculosis (TB) patients. Heteroresistance of MTB is considered a preliminary stage to full resistance. The present study aimed to determine the heteroresistance in Mycobacterium tuberculosis in Tabuk province, in the north of the Kingdom of Saudi Arabia.
GenoType MTBDRplus assay was used to determine mutations associated with isoniazid and rifampicin resistance.
A total number of 46 confirmed M. tuberculosis positive sputum samples were scanned for heteroresistance. The present study revealed 3 (6.5%) heteroresistant mutations to either rpoB gene alone, 2 (4.4%) to rpoB and 1 (2.2%) to inhA genes.
The detection of heteroresistant mutations could guide the initiation of an appropriate regimen of treatment.
异耐药结核分枝杆菌（MTB）定义为从结核病（TB）患者的单个临床标本中分离出的一组对药物敏感和耐药的细菌。MTB 的异耐药性被认为是完全耐药的早期阶段。本研究旨在确定沙特阿拉伯北部塔布克省结核分枝杆菌的异耐药性。
采用 GenoType MTBDRplus 检测法检测与异烟肼和利福平耐药相关的突变。
对 46 份确诊的结核分枝杆菌阳性痰样本进行了异耐药性扫描。本研究显示，rpoB 基因单独存在 3（6.5%）种异耐药突变，rpoB 和 inhA 基因各存在 2（4.4%）和 1（2.2%）种异耐药突变。
检测异耐药突变可指导启动适当的治疗方案。
