从哺乳动物中分离出的H9N2病毒在小鼠体内的复制水平高于禽源病毒。

H9N2 Viruses Isolated From Mammals Replicated in Mice at Higher Levels Than Avian-Origin Viruses.

作者信息

Sun Haiwei, Wang Kai, Yao Wei, Liu Qinfang, Yang Jianmei, Teng Qiaoyang, Li Xuesong, Li Zejun, Chen Hongjun

机构信息

Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.

Animal Influenza Virus Ecology and Pathogenesis Innovation Team, The Agricultural Science and Technology Innovation Program, Shanghai, China.

出版信息

Front Microbiol. 2019 Mar 11;10:416. doi: 10.3389/fmicb.2019.00416. eCollection 2019.

H9N2 subtype influenza A virus (IAV) has more than 20 genotypes that are able to cross species barriers and expand from birds to mammals and humans. To better understand the impact of different H9N2 genotypes and their characteristics, five H9N2 viruses from different hosts including chickens, geese, pigs, mink, and humans representing the B69 88(Gs/14, Ck/15, and Mi/14), B35 (Sw/08) and G9 genotypes (Hu/04) were infected in chicken and mice. In mice, mammal-origin viruses replicated at higher levels in the lungs compared to avian viruses. The goose-virus replicated at the lowest levels indicating poor adaptation. Increased pro-inflammatory cytokines were positively correlated with viral loads in the lung. In chickens, all viruses were excreted from cloacal and/or oropharyngeal swabs. Interestingly, Mink-origin virus exhibited higher virulence and replication in mice and chickens. Our data indicate that mammal-origin H9N2 viruses are more adapted and virulent in mice than the avian-origin viruses.

H9N2亚型甲型流感病毒（IAV）有20多种基因型，能够跨越物种屏障，从鸟类传播到哺乳动物和人类。为了更好地了解不同H9N2基因型的影响及其特征，将来自鸡、鹅、猪、水貂和人类等不同宿主的5种H9N2病毒（代表B69 88（Gs/14、Ck/15和Mi/14）、B35（Sw/08）和G9基因型（Hu/04））感染鸡和小鼠。在小鼠中，与禽源病毒相比，哺乳动物源病毒在肺中的复制水平更高。鹅源病毒复制水平最低，表明适应性较差。促炎细胞因子增加与肺中的病毒载量呈正相关。在鸡中，所有病毒都从泄殖腔和/或口咽拭子中排出。有趣的是，水貂源病毒在小鼠和鸡中表现出更高的毒力和复制能力。我们的数据表明，与禽源病毒相比，哺乳动物源H9N2病毒在小鼠中更易适应且毒力更强。