通过对巴西人类粪便样本进行深度测序发现甜瓜内病毒。
Discovery of Cucumis melo endornavirus by deep sequencing of human stool samples in Brazil.
作者信息
da Costa Antonio Charlys, Leal Elcio, Gill Danielle, Milagres Flavio Augusto de Pádua, Komninakis Shirley Vasconcelos, Brustulin Rafael, Teles Maria da Aparecida Rodrigues, Lobato Márcia Cristina Alves Brito Sayão, das Chagas Rogério Togisaki, Abrão Maria de Fátima Neves Dos Santos, Soares Cassia Vitória de Deus Alves, Deng Xutao, Delwart Eric, Sabino Ester Cerdeira, Luchs Adriana
机构信息
Institute of Tropical Medicine, University of São Paulo, Av. Dr. Enéas de Carvalho Aguiar, no 470, São Paulo, São Paulo, 05403-000, Brazil.
LIM/46, Faculty of Medicine, University of São Paulo, São Paulo, Brazil.
出版信息
Virus Genes. 2019 Jun;55(3):332-338. doi: 10.1007/s11262-019-01648-0. Epub 2019 Mar 26.
The nearly complete genome sequences of two Cucumis melo endornavirus (CmEV) strains were obtained using deep sequencing while investigating fecal samples for the presence of gastroenteritis viruses. The Brazilian CmEV BRA/TO-23 (aa positions 116-5027) and BRA/TO-74 (aa positions 26-5057) strains were nearly identical to the reference CmEV CL-01 (USA) and SJ1 (South Korea) strains, showing 97% and 98% of nucleotide and amino acid identity, respectively. Endornaviruses are not known to be associated with human disease and their presence may simply reflect recent dietary consumption. Metagenomic analyses offered an opportunity to identify for the first time in Brazil a newly described endornavirus species.
在对粪便样本进行肠胃炎病毒检测时，通过深度测序获得了两种甜瓜内病毒（CmEV）毒株几乎完整的基因组序列。巴西的CmEV BRA/TO-23毒株（氨基酸位置116 - 5027）和BRA/TO-74毒株（氨基酸位置26 - 5057）与参考的美国CmEV CL-01毒株和韩国SJ1毒株几乎相同，核苷酸和氨基酸同一性分别为97%和98%。目前尚不清楚内病毒与人类疾病有关，它们的存在可能仅仅反映了近期的饮食摄入情况。宏基因组分析为在巴西首次鉴定出一种新描述的内病毒物种提供了机会。
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