Hillyer G V, Serrano A E
J Helminthol. 1986 Sep;60(3):173-8. doi: 10.1017/s0022149x00026055.
A Fasciola hepatica tegument antigen preparation was obtained from intact adult worms by solubilization with a non-ionic detergent, Nonidet P-40. This antigen preparation contains antigens useful for the serodiagnosis of infection with this parasite. However, the antigen preparation is inadequate for use in the enzyme-linked immunosorbent assay (ELISA). The present work demonstrates that fractionation by demulsification of this antigen preparation with ammonium sulphate results in a soluble aqueous phase which contains F. hepatica serodiagnostic antigens which can then be applied to the ELISA. This F. hepatica tegument antigen preparation when used in the ELISA can detect rabbit fascioliasis two weeks after infection, with antibody levels peaking by 10 to 12 weeks of infection.
通过用非离子去污剂Nonidet P - 40溶解完整的成虫获得肝片吸虫体表抗原制剂。该抗原制剂包含可用于该寄生虫感染血清学诊断的抗原。然而，该抗原制剂不足以用于酶联免疫吸附测定（ELISA）。目前的工作表明，用硫酸铵对该抗原制剂进行破乳分级分离会产生一个可溶性水相，其中含有可用于ELISA的肝片吸虫血清学诊断抗原。这种肝片吸虫体表抗原制剂用于ELISA时，可在感染后两周检测到兔肝片吸虫病，抗体水平在感染10至12周时达到峰值。
