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肝片吸虫体表抗原的分离

Isolation of Fasciola hepatica tegument antigens.

作者信息

Hillyer G V

出版信息

J Clin Microbiol. 1980 Nov;12(5):695-9. doi: 10.1128/jcm.12.5.695-699.1980.

DOI:10.1128/jcm.12.5.695-699.1980
PMID:6792216
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC273674/
Abstract

Fasciola hepatica tegument antigens were isolated from intact worms in the cold by using Nonidet P-40. Proof of the tegumental nature of the antigens was shown by the peroxidase-antiperoxidase immunocytochemical technique at the light microscope level. The potential of F. hepatica tegument antigens for the immunodiagnosis of rabbit and human fascioliasis was shown by Ouchterlony immunodiffusion, although cross-reactivity was evident in one of six serum samples from patients infected with Schistosoma mansoni. A genus-specific Fasciola antigen was found in F. hepatica tegument. Finally, F. hepatica tegument contained antigens which protected mice from challenge infection with S. mansoni.

摘要

用诺乃洗涤剂P - 40在低温下从完整的肝片吸虫中分离出其体表抗原。通过过氧化物酶 - 抗过氧化物酶免疫细胞化学技术在光学显微镜水平证实了这些抗原的体表性质。尽管在六份曼氏血吸虫感染患者的血清样本中有一份出现明显交叉反应，但通过双向免疫扩散试验表明肝片吸虫体表抗原在兔和人类肝片吸虫病免疫诊断中的潜力。在肝片吸虫体表发现了一种属特异性的片形吸虫抗原。最后，肝片吸虫体表含有能保护小鼠免受曼氏血吸虫攻击感染的抗原。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5340/273674/b86a60170c3e/jcm00172-0084-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5340/273674/272226f9cd78/jcm00172-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5340/273674/bf5531a9f5e7/jcm00172-0082-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5340/273674/f6cc9b36db58/jcm00172-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5340/273674/1f871aa3a6c5/jcm00172-0083-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5340/273674/088328190911/jcm00172-0083-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5340/273674/3a6ec4e910ee/jcm00172-0083-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5340/273674/b86a60170c3e/jcm00172-0084-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5340/273674/272226f9cd78/jcm00172-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5340/273674/bf5531a9f5e7/jcm00172-0082-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5340/273674/f6cc9b36db58/jcm00172-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5340/273674/1f871aa3a6c5/jcm00172-0083-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5340/273674/088328190911/jcm00172-0083-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5340/273674/3a6ec4e910ee/jcm00172-0083-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5340/273674/b86a60170c3e/jcm00172-0084-a.jpg

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Isolation of Fasciola hepatica tegument antigens.肝片吸虫体表抗原的分离
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本文引用的文献

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[Isolation of a specific antigen from the large liver fluke (Fasciola hepatica L.)].[从大片吸虫（肝片吸虫）中分离出一种特异性抗原]
C R Acad Hebd Seances Acad Sci D. 1967 Jul 31;265(5):466-9.
2
The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes.免疫组织化学的未标记抗体酶法：可溶性抗原-抗体复合物（辣根过氧化物酶-抗辣根过氧化物酶）的制备、性质及其在螺旋体鉴定中的应用
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Schistosoma mansoni: reduced worm burdens in mice immunized with isolated Fasciola hepatica antigens.
Evaluation and characterization of Fasciola hepatica tegument protein extract for serodiagnosis of human fascioliasis.
用于人体肝片吸虫病血清学诊断的肝片吸虫体表蛋白提取物的评估与特性分析
Clin Vaccine Immunol. 2012 Nov;19(11):1870-8. doi: 10.1128/CVI.00487-12. Epub 2012 Sep 26.
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Biochemical characterization and differential expression of a 16.5-kilodalton tegument-associated antigen from the liver fluke Fasciola hepatica.肝片吸虫16.5千道尔顿被膜相关抗原的生化特性及差异表达
Clin Vaccine Immunol. 2012 Mar;19(3):325-33. doi: 10.1128/CVI.05501-11. Epub 2012 Jan 25.
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The Fasciola hepatica tegumental antigen suppresses dendritic cell maturation and function.肝片吸虫体表抗原抑制树突状细胞的成熟和功能。
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Laurell crossed immunoelectrophoresis and affinity chromatography for the purification of a parasite antigen.劳雷尔交叉免疫电泳和亲和层析用于纯化一种寄生虫抗原。
J Immunol Methods. 1978;20:385-90. doi: 10.1016/0022-1759(78)90273-9.
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The formation and turnover of the membranocalyx on the tegument of Schistosoma mansoni.
Parasitology. 1977 Feb;74(1):61-71. doi: 10.1017/s0031182000047533.
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Fasciola hepatica: ultrastructure and histochemistry of the glycocalyx of the tegument.
Exp Parasitol. 1976 Feb;39(1):119-34. doi: 10.1016/0014-4894(76)90019-9.
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Immunity in Schistosoma mansoni using antigens of Fasciola hepatica isolated by concanavalin A affinity chromatography.利用伴刀豆球蛋白A亲和层析法分离的肝片吸虫抗原对曼氏血吸虫进行免疫研究。
Infect Immun. 1979 Dec;26(3):802-7. doi: 10.1128/iai.26.3.802-807.1979.
8
Can we vaccinate against schistosomes?我们能接种血吸虫疫苗吗？
Fed Proc. 1976 Dec;35(14):2568-71.