Hoppe Travis, Minton Allen P
Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, United States.
Front Mol Biosci. 2019 Mar 13;6:10. doi: 10.3389/fmolb.2019.00010. eCollection 2019.
A general thermodynamic formulation of the effect of hard and soft non-specific intermolecular interactions upon reaction equilibria is summarized. A highly simplified quantitative model for non-specific intermolecular interaction is introduced. This model is used to illustrate how the magnitudes of attractive and repulsive components of the overall intermolecular interaction, and the balance between them, influence the concentration-dependent properties of a highly concentrated solution of a single macromolecular solute. The properties calculated using the results of computer simulation and an approximate analytical model are found to agree qualitatively with the results of experimental measurements on protein solutions over a broad range of concentration.
总结了硬和软非特异性分子间相互作用对反应平衡影响的一般热力学公式。引入了一个高度简化的非特异性分子间相互作用定量模型。该模型用于说明整体分子间相互作用的吸引和排斥成分的大小及其之间的平衡如何影响单一高分子溶质高浓度溶液的浓度依赖性性质。发现使用计算机模拟结果和近似分析模型计算得到的性质在很宽的浓度范围内与蛋白质溶液实验测量结果定性一致。
