Costanzo Manuela, Malatesta Manuela
University of Verona, Department of Neurosciences, Biomedicine and Movement Sciences, Section of Anatomy and Histology.
Eur J Histochem. 2019 Mar 28;63(1):3026. doi: 10.4081/ejh.2019.3026.
Transmission electron microscopy is the technique of choice to visualize the spatial relationships between nanoconstructs and cells and especially to monitor the uptake process of nanomaterials. It is therefore crucial that the cell surface be preserved in its integrity, to obtain reliable ultrastructural evidence: the plasmalemma represents the biological barrier the nanomaterials have to cross, and the mode of membrane-nanoconstruct interaction is responsible for the intracellular fate of the nanomaterials. In this paper, we describe a simple and inexpensive method to process cell monolayers for ultrastructural morphology and immunocytochemistry, ensuring consistent preservation of the cell surface and of the occurring interactions with nanoparticles of different chemical composition.
透射电子显微镜是观察纳米结构与细胞之间空间关系，尤其是监测纳米材料摄取过程的首选技术。因此，保持细胞表面的完整性至关重要，以便获得可靠的超微结构证据：质膜代表纳米材料必须跨越的生物屏障，膜与纳米结构的相互作用方式决定了纳米材料在细胞内的命运。在本文中，我们描述了一种简单且经济的方法，用于处理细胞单层以进行超微结构形态学和免疫细胞化学研究，确保细胞表面以及与不同化学成分纳米颗粒之间发生的相互作用得到一致的保存。
