Taylor B J, Fewell J E, Kearns G L, Hill D E
Pediatr Res. 1986 Sep;20(9):834-7. doi: 10.1203/00006450-198609000-00005.
We investigated the effect of cromolyn sodium, a mast cell stabilizing agent, on the pulmonary vascular response to alveolar hypoxia in six chronically instrumented lambs aged 9 to 11 days. Each lamb was instrumented on day 6 or 7 for measurements of systemic arterial, pulmonary arterial and left atrial pressures, and pulmonary blood flow. The animals were allowed to recover from surgery at least 3 days before they were studied. Each animal was studied twice, once with a cromolyn sodium infusion and once with a normal saline infusion (placebo). These paired experiments were separated by 24 h. Physiologic measurements were made during a 1-min predose control period, after an 8-min drug or placebo infusion, and after a 15-min period of alveolar hypoxia. Cromolyn sodium infusion alone did not affect baseline cardiovascular variables. Alveolar hypoxia following placebo infusion produced an increase in pulmonary arterial pressure and pulmonary vascular resistance; these responses were blocked in the animals given cromolyn sodium prior to induction of hypoxia. These results show that cromolyn sodium blocks the pulmonary vascular response to hypoxia and provide indirect evidence that mast cell degranulation, with subsequent release of vasoactive agents, mediates the pulmonary vascular response to hypoxia in newborn lambs.
我们研究了肥大细胞稳定剂色甘酸钠对6只9至11日龄长期植入仪器的羔羊肺血管对肺泡缺氧反应的影响。每只羔羊在第6天或第7天植入仪器，用于测量体动脉、肺动脉和左心房压力以及肺血流量。在进行研究前，让动物从手术中恢复至少3天。每只动物进行两次研究，一次输注色甘酸钠，一次输注生理盐水（安慰剂）。这些配对实验间隔24小时。在1分钟的给药前对照期、8分钟的药物或安慰剂输注后以及15分钟的肺泡缺氧期后进行生理测量。单独输注色甘酸钠不影响基线心血管变量。安慰剂输注后的肺泡缺氧导致肺动脉压和肺血管阻力增加；在缺氧诱导前给予色甘酸钠的动物中，这些反应受到阻断。这些结果表明，色甘酸钠可阻断肺血管对缺氧的反应，并间接证明肥大细胞脱颗粒以及随后血管活性物质的释放介导了新生羔羊肺血管对缺氧的反应。
