Department of Clinical Analysis and Biomedicine, State University of Maringá, Maringá, Paraná, Brazil.
Department of Chemistry, State University of Maringa, Maringá, Brazil.
Photochem Photobiol. 2019 Sep;95(5):1230-1238. doi: 10.1111/php.13104. Epub 2019 Apr 16.
This study evaluated the rose bengal- and erythrosine-mediated photoinactivation against Salmonella Typhimurium and Staphylococcus aureus planktonic and sessile cells using green LED as a light source. The free-living or 2-day-old biofilm cells were treated with different concentrations of the photosensitizing agents and subjected to irradiation. Only 5 min photosensitization with rose bengal at 25 nmol L and 75 μmol L completely eliminated S. aureus and S. Typhimurium planktonic cells, respectively. Erythrosine at 500 nmol L and 5 min of light exposure also reduced S. aureus planktonic cells to undetectable levels. Eradication of S. aureus biofilms was achieved when 500 μmol L of erythrosine or 250 μmol L of rose bengal was combined with 30 min of irradiation. Scanning electron microscopy allowed the observation of morphological changes in planktonic cells and disruption of the biofilm architecture after photodynamic treatment. The overall data demonstrate that rose bengal and erythrosine activated by green LED may be a targeted strategy for controlling foodborne pathogens in both planktonic and sessile states.
本研究评估了使用绿光 LED 作为光源时，玫瑰红和赤藓红介导的光动力灭活对鼠伤寒沙门氏菌和金黄色葡萄球菌浮游和固着细胞的效果。用不同浓度的光敏剂处理自由生活或 2 天龄的生物膜细胞，并进行照射。仅需 5 分钟的 25 nmol L 玫瑰红和 75 μmol L 玫瑰红敏化处理即可分别完全消除金黄色葡萄球菌和鼠伤寒沙门氏菌浮游细胞。500 nmol L 赤藓红和 5 分钟的光照也可将金黄色葡萄球菌浮游细胞减少到无法检测的水平。当将 500 μmol L 的赤藓红或 250 μmol L 的玫瑰红与 30 分钟的照射相结合时，可实现对金黄色葡萄球菌生物膜的根除。扫描电子显微镜允许观察浮游细胞的形态变化和光动力处理后生物膜结构的破坏。总体数据表明，绿光 LED 激活的玫瑰红和赤藓红可能是控制浮游和固着状态食源性病原体的靶向策略。
