Yassunaka Natália Norika, Freitas Camila Fabiano de, Rabello Bruno Ribeiro, Santos Patrícia Regina, Caetano Wilker, Hioka Noboru, Nakamura Tania Ueda, Abreu Filho Benício Alves de, Graton Mikcha Jane Martha
Programa de Pós-Graduação em Ciência de Alimentos, Universidade Estadual de Maringá, Maringá, PR, Brazil.
Curr Microbiol. 2015 Aug;71(2):243-51. doi: 10.1007/s00284-015-0827-5. Epub 2015 Apr 30.
The purpose of the present study was to evaluate the efficacy of photodynamic inactivation (PDI) mediated by erythrosine (ERY) and its ester derivatives erythrosine methyl ester (ERYMET) and erythrosine butyl ester (ERYBUT) on foodborne pathogens and spoilage bacteria. We evaluated Staphylococcus aureus ATCC 25923, Aeromonas hydrophila ATCC 7966, Salmonella enterica serotype Typhimurium ATCC 14028, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 27853. The toxicity of all of the compounds was assessed in VERO cells. PDI mediated by ERY and its derivatives combined with a light-emitting diode was performed at different concentrations and exposure times. S. aureus was more photosensitive than Gram-negative bacteria to ERY, ERYMET, and ERYBUT. The ERY-mediated PDI of S. aureus induced a significant reduction of 4.0 log CFU/ml at a light dose of 40 J/cm(2). ERYMET and ERYBUT at lower light doses than ERY completely eradicated S. aureus. When photoirradiated with ERY at light doses of 156 and 234 J/cm(2), A. hydrophila was completely eradicated. ERYBUT was more efficient in the PDI of A. hydrophila than ERYMET, even at 1 x 10(-5) M and lower light doses. Salmonella Typhimurium, E. coli, and P. aeruginosa required higher concentrations of photosensitizers to reduce cell survival. ERYBUT and ERY may be promising photosensitizing agents against A. hydrophila and S. aureus. They were effective at reducing bacterial counts at nontoxic concentrations. The photoinactivation rate of the evaluated bacteria decreased in the following order: S. aureus > A. hydrophila > E. coli > S. Typhimurium > P. aeruginosa.
本研究的目的是评估赤藓红（ERY）及其酯衍生物赤藓红甲酯（ERYMET）和赤藓红丁酯（ERYBUT）介导的光动力灭活（PDI）对食源性病原体和腐败菌的效果。我们评估了金黄色葡萄球菌ATCC 25923、嗜水气单胞菌ATCC 7966、鼠伤寒沙门氏菌ATCC 14028、大肠杆菌ATCC 25922和铜绿假单胞菌ATCC 27853。在VERO细胞中评估了所有化合物的毒性。ERY及其衍生物与发光二极管联合介导的PDI在不同浓度和暴露时间下进行。金黄色葡萄球菌对ERY、ERYMET和ERYBUT的光敏性高于革兰氏阴性菌。在40 J/cm(2)的光剂量下，ERY介导的金黄色葡萄球菌PDI导致细菌数量显著减少4.0 log CFU/ml。ERYMET和ERYBUT在比ERY更低的光剂量下完全根除了金黄色葡萄球菌。当用156和234 J/cm(2)的光剂量照射ERY时，嗜水气单胞菌被完全根除。即使在1×10(-5) M及更低的光剂量下，ERYBUT在嗜水气单胞菌的PDI中也比ERYMET更有效。鼠伤寒沙门氏菌、大肠杆菌和铜绿假单胞菌需要更高浓度的光敏剂来降低细胞存活率。ERYBUT和ERY可能是针对嗜水气单胞菌和金黄色葡萄球菌的有前景的光敏剂。它们在无毒浓度下能有效降低细菌数量。所评估细菌的光灭活率按以下顺序降低：金黄色葡萄球菌>嗜水气单胞菌>大肠杆菌>鼠伤寒沙门氏菌>铜绿假单胞菌。
