Santos Ana Carla da Silva, Trindade José Vinícius Correia, Lima Cristiano Souza, Barbosa Renan do Nascimento, da Costa Antonio Félix, Tiago Patricia Vieira, de Oliveira Neiva Tinti
a Departamento de Micologia, Universidade Federal de Pernambuco, Av. Professor Moraes Rego 1235 , Cidade Universitária , Recife , Pernambuco , 50670-901 , Brazil.
b Departamento de Fitotecnia, Universidade Federal do Ceará , Av. Mister Hull 2977, Presidente Kennedy , Fortaleza , Ceará , 60356-001 , Brazil.
Mycologia. 2019 Mar-Apr;111(2):244-259. doi: 10.1080/00275514.2019.1573047. Epub 2019 Mar 29.
Based on morphological and molecular phylogenetic markers and the fertility of sexual crosses, two novel species of Fusarium associated with Dactylopius opuntiae (Hemiptera: Dactylopiidae) and Aleurocanthus woglumi (Hemiptera: Aleyrodidae) from northeastern Brazil are described as Fusarium caatingaense and F. pernambucanum. Partial sequences of five loci were generated for 29 entomopathogenic Fusarium isolates. Multilocus phylogenetic analyses demonstrated that F. caatingaense and F. pernambucanum belong to the Incarnatum clade of the Fusarium incarnatum-equiseti species complex (FIESC). These species displayed common morphological characters such as the production of various types of aerial conidia formed on monophialides and polyphialides and differ from each other mainly in the dimensions and morphology of their sporodochial conidia. Mating type polymerase chain reaction (PCR) revealed 17 MAT1-1 isolates and 12 MAT1-2 isolates, all of them heterothallic. Fertile perithecia were produced in 4.2% of infraspecific crosses of F. caatingaense and in 13.3% of infraspecific crosses of F. pernambucanum after 2-3 wk. Crosses between F. caatingaense and F. pernambucanum did not result in fertile perithecia. We demonstrate the existence of a sexual stage in species of the Incarnatum clade and describe the morphological characters of these sexual morphs for the first time. These results suggest that previously unknown sexual cycles contribute to the high genetic diversity within FIESC.
基于形态学和分子系统发育标记以及有性杂交的育性，来自巴西东北部与仙人掌胭脂虫（半翅目：胭蚧科）和可可刺粉虱（半翅目：粉虱科）相关的两种镰刀菌新物种被描述为卡廷加镰刀菌和伯南布哥镰刀菌。对29株昆虫病原镰刀菌分离株进行了5个基因座的部分序列测定。多位点系统发育分析表明，卡廷加镰刀菌和伯南布哥镰刀菌属于镰刀菌-轮枝菌复合种（FIESC）的肉色镰刀菌分支。这些物种表现出共同的形态特征，如在单瓶梗和多瓶梗上产生各种类型的气生分生孢子，主要区别在于它们分生孢子座分生孢子的尺寸和形态。交配型聚合酶链反应（PCR）显示有17株MAT1-1分离株和12株MAT1-2分离株，它们均为异宗配合。在2-3周后，卡廷加镰刀菌种内杂交中有4.2%产生了可育子囊壳，伯南布哥镰刀菌种内杂交中有13.3%产生了可育子囊壳。卡廷加镰刀菌和伯南布哥镰刀菌之间的杂交未产生可育子囊壳。我们首次证明了肉色镰刀菌分支物种中有性阶段的存在，并描述了这些有性形态的形态特征。这些结果表明，以前未知的有性周期促成了FIESC内的高遗传多样性。
