Institute of Chemistry, Health and Food Sciences, Jan Dlugosz University in Czestochowa, 42-200 Czestochowa, Poland.
Institute for Engineering of Polymer Materials and Dyes, 87-100 Torun, Poland.
Carbohydr Polym. 2019 Jun 15;214:124-130. doi: 10.1016/j.carbpol.2019.03.034. Epub 2019 Mar 14.
Hydrophobic starch derivatives were obtained by biocatalysed esterification with hydrolysates from high oleic vegetable oils. First, the pure rapeseed oil or waste rapeseed oil, was hydrolysed with a Thermomyces lanuginosus lipase in the buffer solution form. Next, purified hydrolysates were used as donors of the acyl group for the esterification of potato starch after being pregelatinised in ionic liquid - 1-buthyl-3-methylimidazolium chloride. The esterification was catalysed by the same fungal lipase but immobilised on a polymer carrier and conducted in the anhydrous ternary reaction system: hydrophilic ionic liquid - non-ionic surfactant - hydrophobic higher fatty acids. The products have been identified and characterised using the following methods: degree of substitution (DS) by volumetric and elemental analysis method, FTIR and NMR spectroscopies, XRD and SEM. The proposed method can lead to the synthesis of more water-resistant starch derivatives as well as provide a possibility for recycling waste oils from the food industry.
通过生物催化酯交换反应，用高油酸蔬菜油水解产物制备了疏水淀粉衍生物。首先，采用Thermomyces lanuginosus 脂肪酶在缓冲溶液中水解纯油菜籽油或废油菜籽油。然后，将经过离子液体-1-丁基-3-甲基咪唑氯化物预糊化的纯化水解产物用作酰基供体，用于酯化马铃薯淀粉。酯化反应由相同的真菌脂肪酶催化，但固定在聚合物载体上，并在无水三元反应体系中进行：亲水性离子液体-非离子表面活性剂-疏水性高级脂肪酸。使用以下方法鉴定和表征产物：通过容量和元素分析方法、FTIR 和 NMR 光谱、XRD 和 SEM 测定取代度 (DS)。所提出的方法可以导致合成更耐水的淀粉衍生物，并为食品工业中的废油回收提供可能性。
