Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing Research Center for Pharmaceutical Engineering, School of Pharmacy, Chongqing Medical University, Chongqing, 400016, PR China.
Chongqing Key Laboratory of Ultrasound Molecular Imaging, Institute of Ultrasound Imaging, Chongqing Medical University, Chongqing, 400016, PR China.
Carbohydr Polym. 2019 Jun 15;214:221-233. doi: 10.1016/j.carbpol.2019.03.043. Epub 2019 Mar 14.
This study has developed a versatile nano-system with the combined advantages of photothermal effect, active tumor-targeting, temperature-sensitive drug release, and photoacoustic imaging. The nano-system consists of the core of the phase change material (PCM), the outer polypyrrole (PPY) shell and the hyaluronic acid (HA) modified in the PPY shell. The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA) were spherical with a mean diameter of about 232.7 nm. In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy. DTX/PPN@PPY@HA shows good photothermal effects and temperature-sensitive drug release. In addition, cellular experiments showed that DTX/PPN@PPY@HA could be efficiently internalized into tumor cells and produce significant cytotoxicity with the help of near-infrared (NIR) laser. Furthermore, the remarkable inhibition of DTX/PPN@PPY@HA against tumor growth was achieved in 4T1 tumor-bearing mice model.
本研究开发了一种具有光热效应、主动肿瘤靶向、温度敏感药物释放和光声成像相结合优势的多功能纳米系统。该纳米系统由相变材料（PCM）核心、聚吡咯（PPY）外壳和在 PPY 壳中修饰的透明质酸（HA）组成。所得到的复合纳米粒子（表示为 DTX/PPN@PPY@HA）呈球形，平均直径约为 232.7nm。体内和体外光声成像实验表明，DTX/PPN@PPY@HA 是一种有效的光声对比剂，可用于肿瘤区域的精确定位和光热化疗的实时指导。DTX/PPN@PPY@HA 表现出良好的光热效应和温度敏感药物释放。此外，细胞实验表明，在近红外（NIR）激光的帮助下，DTX/PPN@PPY@HA 可以有效地被肿瘤细胞内化，并产生显著的细胞毒性。此外，在 4T1 荷瘤小鼠模型中，DTX/PPN@PPY@HA 对肿瘤生长的显著抑制作用得以实现。
