Grupo de Inmunología Celular e Inmunogenética, Sede de Investigación Universitaria (SIU), Universidad de Antioquia (UDEA), Calle 70 No. 52-21, Medellín, Colombia.
Grupo de Física del Estado Sólido, Sede de Investigación Universitaria (SIU), Universidad de Antioquia (UDEA), Colombia.
Cell Immunol. 2019 Apr;338:51-62. doi: 10.1016/j.cellimm.2019.03.005. Epub 2019 Mar 26.
Poly(acrylic acid)-Coated Iron Oxide Nanoparticles (PAC-IONs) did not compromise the viability of mononuclear cells and potentially interact with cells through scavenger receptors. This study evaluated: 1) The capacity of the PAC-IONs to induce platelet activation and aggregation, and 2) The effect of the PAC-IONs in two functions of Monocyte-Derived Macrophages (MDMs) when differentiated in their presence; that is, the removal of apoptotic cells (ACs) and the levels of cytokines induced by Lipopolysaccharide (LPS) and the ACs. The PAC-IONs did not affect the platelet activation but antagonized their aggregation. On the other hand, the differentiation of MDMS in the presence of PAC-IONs did not inhibit the ability of these cells to phagocytose latex beads but decreased the number of apoptotic bodies internalized by them. MDMs differentiated in the presence of PAC-IONs and stimulated with LPS or ACs exhibited an overall decrease of the cytokine levels. The altered synthesis of cytokines could be attributed to a high production of Reactive Oxygen Species (ROS) caused by the increase in the intracellular iron content. The effect of the PAC-IONs on the cell cycle of U937 and Jurkat cells was also studied; there was not either cell accumulation in any phase of the cell cycle or changes in the DNA content. It is clear that PAC-IONs affect neither the viability nor compromise some cellular functions. However, they could alter the functioning of the immune system; therefore, in the case of being used as a diagnostic tool, their permanence in the body should be considered.
聚（丙烯酸）-包覆的氧化铁纳米粒子（PAC-IONs）不会影响单核细胞的活力，并可能通过清道夫受体与细胞相互作用。本研究评估了：1）PAC-IONs 诱导血小板活化和聚集的能力，以及 2）在存在 PAC-IONs 的情况下，单核细胞衍生的巨噬细胞（MDMs）分化时，PAC-IONs 对两种功能的影响；也就是说，清除凋亡细胞（ACs）和由脂多糖（LPS）和 ACs 诱导的细胞因子的水平。PAC-IONs 不会影响血小板的活化，但会拮抗其聚集。另一方面，在 PAC-IONs 存在的情况下分化的 MDMS 不会抑制这些细胞吞噬乳胶珠的能力，但会减少它们内化的凋亡小体的数量。在 PAC-IONs 存在下分化并受 LPS 或 ACs 刺激的 MDMs 表现出细胞因子水平总体下降。细胞因子合成的改变可能归因于细胞内铁含量增加导致的活性氧（ROS）产生增加。还研究了 PAC-IONs 对 U937 和 Jurkat 细胞细胞周期的影响；细胞周期的任何阶段都没有细胞积累或 DNA 含量发生变化。显然，PAC-IONs 既不影响细胞活力也不损害某些细胞功能。然而，它们可能会改变免疫系统的功能；因此，如果将其用作诊断工具，则应考虑其在体内的持久性。
