College of Life Science and Technology, Guangxi University, Nanning 530004, Guangxi, China.
Viticulture and Wine Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China.
Fungal Biol. 2019 Apr;123(4):283-289. doi: 10.1016/j.funbio.2018.11.014. Epub 2019 Feb 8.
The two-harvest-per-year farming system allow table grape to be harvested a year both in summer and winter in southern China. Herein, we used high-throughput sequencing to investigate the diversity of fungi on grape fruits surface during the ripening process in summer and winter at subtropical Nanning region, Guangxi, China. The results showed that 23 fungal species existed in all samples. Among them, the five most dominant species were Cladosporium ramotenellum, Pseudozyma aphidis, Gyrothrix spp., Gibberella intricans and Acremonium alternatum, with abundance from 61.62 % to 91.26 %. Analysis using the student's t-test for Shannon index indicated that components of fungal community varied significantly between the two ripening seasons. The dominant genera of core fungal community were Cladosporium, Gyrothrix, Paramycosphaerella, Acremonium, Penicillium and Tilletiopsis in the summer and Cladosporium, Pseudozyma, Gibberella, Colletotrichum, Sporobolomyces, Rhodosporidium, Alternaria and Aspergillus in the winter. Overall, fungi diversity on grape fruits surface at Nanning showed significantly differences between different ripening seasons. Our results ennrich the understanding of epiphytic communities of grape fruits in subtropics.
一年两收的种植制度使中国南方的葡萄在夏季和冬季都能达到可收获的状态。在此，我们使用高通量测序技术研究了中国广西亚热带南宁地区夏季和冬季葡萄果实表面真菌在成熟过程中的多样性。结果表明，所有样本中存在 23 种真菌物种。其中，最占优势的五个物种为枝孢属（Cladosporium ramotenellum）、假尾孢菌属（Pseudozyma aphidis）、枝孢属（Gyrothrix spp.）、藤仓赤霉（Gibberella intricans）和交替单端孢（Acremonium alternatum），丰度为 61.62%-91.26%。通过学生 t 检验对 Shannon 指数的分析表明，两个成熟季节的真菌群落组成存在显著差异。夏季核心真菌群落的优势属为枝孢属（Cladosporium）、枝孢属（Gyrothrix）、拟青霉属（Paramycosphaerella）、单端孢属（Acremonium）、青霉属（Penicillium）和毛壳属（Tilletiopsis），冬季为枝孢属（Cladosporium）、假尾孢菌属（Pseudozyma）、藤仓赤霉（Gibberella intricans）、炭疽菌属（Colletotrichum）、节菱孢属（Sporobolomyces）、红酵母属（Rhodosporidium）、交链孢属（Alternaria）和曲霉属（Aspergillus）。总体而言，南宁葡萄果实表面的真菌多样性在不同的成熟季节之间存在显著差异。我们的研究结果丰富了对亚热带葡萄果实附生群落的认识。
