Carmichael P C, Siyoum N, Chidamba L, Korsten L
Department of Plant and Soil Sciences, University of Pretoria, Hatfield, South Africa.
J Appl Microbiol. 2017 Nov;123(5):1251-1262. doi: 10.1111/jam.13577.
To determine fungal communities that characterize table grapes during berry development.
Two agro-ecologically different table grape commercial farms (site A and B) were used in this study. Samples were collected at full bloom, pea size and mature stages, from three positions (inside centre, eastern and western peripheral ends) per site. Total DNA extraction, Illumina sequencing and analysis of 18 pooled samples for fungal diversity targeting ITS1-2 generated a total of 2 035 933 high-quality sequences. The phylum Ascomycota (77.0%) and Basidiomycota (23.0%) were the most dominant, while the genera, Alternaria (33.1%) and Cladosporium (24.2%) were the overall dominant postharvest decay causing fungi throughout the developmental stages. Inside centre of site A were more diverse at full bloom (3.82) than those at the peripheral ends (<3.8), while at site B, the peripheral ends showed better diversity, particularly the eastern part at both full bloom (3.3) and pea size (3.7).
Fungal population diversity varies with different phenological table grape growth stages and is further influenced by site and vine position within a specific vineyard.
The information on fungal diversity and succession in table grapes during preharvest growth stages is critical in the development of a more targeted control strategy, to improve postharvest quality of table grapes.
确定表征鲜食葡萄浆果发育过程的真菌群落。
本研究使用了两个农业生态不同的鲜食葡萄商业农场（A地和B地）。在盛花期、豌豆大小期和成熟期，从每个地点的三个位置（中心内部、东部和西部外围末端）采集样本。对18个混合样本进行总DNA提取、Illumina测序并针对ITS1-2分析真菌多样性，共产生了2035933条高质量序列。子囊菌门（77.0%）和担子菌门（23.0%）最为占主导地位，而链格孢属（33.1%）和枝孢属（24.2%）是在整个发育阶段导致采后腐烂的总体优势真菌属。A地中心内部在盛花期的多样性（3.82）高于外围末端（<3.8），而在B地，外围末端表现出更好的多样性，尤其是在盛花期（3.3）和豌豆大小期（3.7）的东部。
真菌种群多样性随鲜食葡萄不同物候生长阶段而变化，并在特定葡萄园进一步受到地点和葡萄藤位置的影响。
收获前生长阶段鲜食葡萄真菌多样性和演替的信息对于制定更具针对性的控制策略以提高鲜食葡萄采后质量至关重要。
