Fox R I, Bumol T, Fantozzi R, Bone R, Schreiber R
Arthritis Rheum. 1986 Sep;29(9):1105-11. doi: 10.1002/art.1780290908.
Recent studies have suggested that the induction of HLA-DR antigens on epithelial cells plays an important role in the pathogenesis of autoimmune endocrine-exocrine disorders. We found that salivary gland epithelial cells (i.e., acinar and ductal cells) in salivary gland biopsy specimens from patients with primary Sjögren's syndrome (keratoconjunctivitis sicca) expressed high levels of HLA-DR antigen, which were detected by staining frozen tissue sections with monoclonal antibodies and immunoperoxidase technique. In contrast, salivary gland epithelial cells from normal subjects did not express this antigen. Lymphocytes eluted from the salivary gland biopsy specimens of patients who had Sjögren's syndrome produced a soluble factor that stimulated HLA-DR synthesis by a salivary gland-derived cell line (Sal-1). These tissue culture supernatants contained gamma-interferon, and their ability to induce HLA-DR synthesis was blocked by monoclonal anti-gamma-interferon antibody. These results demonstrate the presence of HLA-DR antigen on salivary gland epithelial cells and suggest that local production of gamma-interferon plays a role in this induction.
近期研究表明，上皮细胞上HLA - DR抗原的诱导在自身免疫性内分泌 - 外分泌疾病的发病机制中起重要作用。我们发现，原发性干燥综合征（角结膜干燥症）患者唾液腺活检标本中的唾液腺上皮细胞（即腺泡细胞和导管细胞）表达高水平的HLA - DR抗原，通过用单克隆抗体和免疫过氧化物酶技术对冰冻组织切片进行染色来检测这些抗原。相比之下，正常受试者的唾液腺上皮细胞不表达这种抗原。从患有干燥综合征患者的唾液腺活检标本中洗脱的淋巴细胞产生一种可溶性因子，该因子可刺激唾液腺来源的细胞系（Sal - 1）合成HLA - DR。这些组织培养上清液含有γ - 干扰素，其诱导HLA - DR合成的能力被单克隆抗γ - 干扰素抗体阻断。这些结果证明唾液腺上皮细胞上存在HLA - DR抗原，并表明γ - 干扰素的局部产生在这种诱导中起作用。
