Department of Environment and Health, Tianjin Institute of Environmental & Operational Medicine, Key Laboratory of Risk Assessment and Control for Environment & Food Safety, Tianjin, 300050, China.
Department of Environment and Health, Tianjin Institute of Environmental & Operational Medicine, Key Laboratory of Risk Assessment and Control for Environment & Food Safety, Tianjin, 300050, China.
Water Res. 2019 Jun 1;156:366-371. doi: 10.1016/j.watres.2019.03.035. Epub 2019 Mar 21.
Adaption to adverse environments plays an important role in bacterial survival and is receiving increasing globe attention now. Here, cultivable chlorine-injured Pseudomonas aeruginosa, produced on the chlorination process, was investigated about their resistance to antibiotics. Then, global transcriptional analyses, quantitative PCR (qPCR) validation and antioxidant enzymes measurement were performed to explore the underlying mechanisms. The results showed that chlorine injury enhanced antibiotic resistance in P. aeruginosa and cultivable chlorine-injured P. aeruginosa exposed to 4 mg/L sodium hypochlorite (half of the lethal dose) improved antibiotic resistance against ceftazidime, chloramphenicol and ampicillin by 1.4-5.6 fold. This increase in antibiotic resistance was not hereditable and over expression of the MexEF-OprN efflux pump resulting from oxidative stress contributed to it. These results demonstrate temporal physiological persistence to antibiotics in cultivable chlorine-injured pathogens, suggesting their survival from adverse environments with antibiotic exposure and thereby posing lasting hazards to human health.
适应不利环境在细菌生存中起着重要作用，目前受到全球越来越多的关注。在这里，我们研究了在氯化过程中产生的可培养氯损伤铜绿假单胞菌对抗生素的耐药性。然后，进行了全局转录分析、定量 PCR（qPCR）验证和抗氧化酶测量，以探讨潜在的机制。结果表明，氯损伤增强了铜绿假单胞菌的抗生素耐药性，并且暴露于 4mg/L 次氯酸钠（半致死剂量）的可培养氯损伤铜绿假单胞菌对头孢他啶、氯霉素和氨苄西林的抗生素耐药性提高了 1.4-5.6 倍。这种抗生素耐药性的增加不是遗传性的，并且氧化应激导致 MexEF-OprN 外排泵的过度表达促成了这种增加。这些结果表明，可培养的氯损伤病原体对抗生素具有暂时的生理持久性，表明它们能够在抗生素暴露的不利环境中生存，从而对人类健康构成持久的威胁。
