氨基酸取代导致 MexT 组成性激活，进而导致铜绿假单胞菌临床分离株 MexEF-OprN 过度表达。
Constitutive Activation of MexT by Amino Acid Substitutions Results in MexEF-OprN Overproduction in Clinical Isolates of Pseudomonas aeruginosa.
机构信息
UMR CNRS 6249 Chrono-Environnement, Laboratoire de Bactériologie, Université de Bourgogne Franche-Comté, Besançon, France.
Research and Development Department, Smaltis SAS, Besançon, France.
出版信息
Antimicrob Agents Chemother. 2018 Apr 26;62(5). doi: 10.1128/AAC.02445-17. Print 2018 May.
Abstract
When overproduced, the multidrug efflux system MexEF-OprN increases the resistance of to fluoroquinolones, chloramphenicol, and trimethoprim. In this work, we demonstrate that gain-of-function mutations in the regulatory gene result in oligomerization of the LysR regulator MexT, constitutive upregulation of the efflux pump, and increased resistance in clinical isolates.
摘要
当过度产生时，多药外排系统 MexEF-OprN 会增加 对氟喹诺酮类、氯霉素和甲氧苄啶的耐药性。在这项工作中，我们证明了调节基因 中的功能获得性突变导致 LysR 调节子 MexT 的寡聚化，外排泵的组成性上调，以及临床分离株的耐药性增加。
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