In this study, the possible cellular effects of tin dioxide (SnO) nanoparticles, together with its bulk form, on mouse dermal fibroblasts (DFs) were revealed using in vitro assays. Particle characterizations were carried out with AFM, Braun-Emmet-Teller, and DLS analyses. The cells were treated with nano and bulk SnO at concentrations of 0.1, 1, 10, 50, and 100 μg/mL for 6, 24, and 48 h. At the end of the exposure periods, the morphology, viability, particle uptake, and membrane leakage statuses of the cells were evaluated. Furthermore, real-time monitoring of cell responses was performed by using an impedance-based label-free system. Findings showed that at concentrations of 0.1-10 μg/mL, cells had similar doubling time to that of control cells (20.4 ± 2.6 h), while the doubling time of cells exposed to 100 μg/mL of nano and bulk SnO increased slightly (P ˃ 0.05) to 25.1 ± 3.9 h and 26.2 ± 5.9 h, respectively. The results indicated that DFs exhibited a similar toxicity response to nano and bulk SnO; thus, 50 and 100 μg/mL of nano and bulk SnO had mild toxic effects on DFs. In conclusion, this study provides information and insight necessary for the safe use of SnO in medical and consumer products.