氧化应激上调参与硫胺素代谢的基因转录。
Oxidative Stress Upregulates the Transcription of Genes Involved in Thiamine Metabolism.
作者信息
Kartal Burcu, Akçay Ahmet, Palabiyik Bedia
机构信息
Department of Genetics and Bioengineering, Faculty of Engineering, Alanya Alaaddin Keykubat University , Antalya , Turkey.
Department of Molecular Biology and Genetics, Institute of Graduate Studies in Science and Engineering, İstanbul University , İstanbul , Turkey.
出版信息
Turk J Biol. 2018 Oct 25;42(5):447-452. doi: 10.3906/biy-1801-51. eCollection 2018.
Thiamine is a major vitamin that acts as a cofactor in energy metabolism in all organisms, as well as in lipid and amino acid metabolisms, and is associated with many diseases. It is known that glucose starvation decreases the intracellular thiamine pool while increasing oxidative stress tolerance. Earlier, in whole genome analysis, we detected major differences in the expression of genes related to thiamine pathway against oxidative stress in Schizosaccharomyces pombe. We investigated the effects of oxidative stress and glucose repression to thiamine pathway in S. pombe by comparing some genes encoding key enzymes of each related pathway at the transcription level. In the present study, we found that the expression of genes related to thiamine biosynthesis and transport (thi2, thi3, and pho1) increased in wild type and ird11 cells grown in thiamine-rich media under oxidative stress induced by H2O2. Based on our findings, we suggested that there might be an important effect of oxidative stress on thiamine biosynthesis and transport.
硫胺素是一种主要的维生素，在所有生物体的能量代谢以及脂质和氨基酸代谢中作为辅助因子发挥作用，并且与许多疾病相关。已知葡萄糖饥饿会减少细胞内硫胺素库，同时增加氧化应激耐受性。此前，在全基因组分析中，我们在粟酒裂殖酵母中检测到硫胺素途径相关基因在抗氧化应激方面的表达存在重大差异。我们通过在转录水平比较每个相关途径的一些关键酶编码基因，研究了氧化应激和葡萄糖阻遏对粟酒裂殖酵母硫胺素途径的影响。在本研究中，我们发现，在富含硫胺素的培养基中生长的野生型和ird11细胞，在H2O2诱导的氧化应激下，与硫胺素生物合成和转运相关的基因（thi2、thi3和pho1）的表达增加。基于我们的发现，我们认为氧化应激可能对硫胺素的生物合成和转运有重要影响。
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