Schweingruber A M, Dlugonski J, Edenharter E, Schweingruber M E
Institute of General Microbiology, University of Bern, Switzerland.
Curr Genet. 1991 Apr;19(4):249-54. doi: 10.1007/BF00355050.
We have investigated the thiamine metabolism in Schizosaccharomyces pombe and shown that: (1) Thiamine-repressible acid phosphate, coded for by the gene pho4, dephosphorylates thiamine phosphates indicating that the enzyme acts as a thiamine phosphate phosphatase. (2) In vivo synthesized thiamine is present intracellularly mainly as thiamine diphosphate. Starving cells for glucose decreases the intracellular thiamine pool. (3) The genes thi2, thi3 and thi4 control thiamine biosynthesis and probably code for thiamine biosynthetic enzymes. Thi3, which is involved in the synthesis of the pyrimidine moiety of the thiamine molecule, is allelic to the thiamine repressible gene nmt1. (4) Thiamine uptake is a thiamine regulated process, probably occurs by active transport and is controlled by the gene ptr1.
我们研究了粟酒裂殖酵母中的硫胺素代谢，并发现：（1）由基因pho4编码的硫胺素可阻遏酸性磷酸酶使硫胺素磷酸酯去磷酸化，这表明该酶作为硫胺素磷酸磷酸酶发挥作用。（2）体内合成的硫胺素在细胞内主要以硫胺素二磷酸的形式存在。使细胞缺乏葡萄糖会减少细胞内硫胺素库。（3）基因thi2、thi3和thi4控制硫胺素生物合成，可能编码硫胺素生物合成酶。参与硫胺素分子嘧啶部分合成的Thi3与硫胺素可阻遏基因nmt1等位。（4）硫胺素摄取是一个受硫胺素调节的过程，可能通过主动运输发生，并受基因ptr1控制。
