Özakin Süleyman, Ince Ebru
Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego , La Jolla, California , USA.
Department of Biology, Faculty of Science, Dicle University , Diyarbakır , Turkey.
Turk J Biol. 2019 Feb 7;43(1):28-36. doi: 10.3906/biy-1807-136. eCollection 2019.
Marine microorganisms are receiving more attention as a promising potential source of new natural products. In the present study, we performed genomic and metabolomic analyses to explore the metabolic potential of the obligate marine actinomycete genus . The genomes of thirty strains were prospected in search of biosynthetic gene clusters including polyketide synthase (PKS), nonribosomal peptide synthetase (NPRS), terpene, indole, lantibiotics, and siderophores. We determined considerable diversity of natural product biosynthetic gene clusters in their genome. There were a total of 1428 putative gene clusters involved in the biosynthesis of various bioactive natural products. Furthermore, 1509 ketosynthase (KS) and condensation (C) domains were detected by using NapDoS belonging to PKS and NRPS genes, respectively. Metabolic profiling was performed by a nontargeted LC-MS/MS approach combined with spectral networking using Global Natural Product Social Molecular Networking (GNPS). Dereplication and tentative identification of natural products were evaluated for common chemical properties and their associated pathways. Significant bioactive natural products such as lomaiviticin C, 7-OH-staurosporine, staurosporine, and cyanosporaside B were determined. More importantly, an unknown glycosylated compound associated with an NRPS/PKS-I hybrid gene cluster in CNY703 was established through chemical and genomic analyses.
海洋微生物作为新天然产物的一个有前景的潜在来源正受到越来越多的关注。在本研究中，我们进行了基因组和代谢组分析，以探索专性海洋放线菌属的代谢潜力。对30株菌株的基因组进行了探测，以寻找生物合成基因簇，包括聚酮合酶（PKS）、非核糖体肽合成酶（NPRS）、萜类、吲哚、羊毛硫抗生素和铁载体。我们确定了它们基因组中天然产物生物合成基因簇的相当大的多样性。共有1428个推定基因簇参与各种生物活性天然产物的生物合成。此外，分别使用属于PKS和NRPS基因的NapDoS检测到1509个酮合酶（KS）和缩合（C）结构域。通过非靶向液相色谱-串联质谱方法结合使用全球天然产物社会分子网络（GNPS）的光谱网络进行代谢谱分析。对天然产物的去重复和初步鉴定进行了常见化学性质及其相关途径的评估。确定了重要的生物活性天然产物，如洛马维汀C、7-羟基星形孢菌素、星形孢菌素和氰孢糖苷B。更重要的是，通过化学和基因组分析确定了一种与CNY703中NRPS/PKS-I杂交基因簇相关的未知糖基化化合物。
