中国新疆宫颈病变患者中16型人乳头瘤病毒E6、E7及长控制区的基因变异
Genetic variations in E6, E7 and the long control region of human papillomavirus type 16 among patients with cervical lesions in Xinjiang, China.
作者信息
Zhe Xiangyi, Xin Huizhen, Pan Zhenzhen, Jin Fuyuan, Zheng Weinan, Li Hongtao, Li Dongmei, Cao Dongdong, Li Ying, Zhang Chunhe, Fu Shaowei, Shao Renfu, Pan Zemin
机构信息
1Key Laboratory of Xinjiang Endemic and Ethnic Diseases/Department of Biochemistry and Molecular Biology, School of Medicine, Shihezi University, Shihezi, 832002 Xinjiang China.
Xinjiang Production and Construction Corps of the Fourth Division Hospital, Yining, 835000 Xinjiang China.
出版信息
Cancer Cell Int. 2019 Mar 20;19:65. doi: 10.1186/s12935-019-0774-5. eCollection 2019.
BACKGROUND
Xinjiang is one of the areas with the highest incidence of cervical cancer in China. Genetic variation in Human papillomavirus type 16 (HPV16) may increase the ability of the virus to mediate carcinogenesis and immune escape, which are risk factors for the progression of cervical cancer. We investigated polymorphism in HPV16 and the distribution of its sub-lineages in the region by analyzing the , and () gene sequences from women with HPV16-positive cervical samples in Xinjiang.
METHODS
A total of 138 cases of cervical lesions and squamous cell carcinoma with infection of HPV16 virus were collected. The and genes and of HPV16 virus were sequenced and compared with the HPV16 European prototype reference and other HPV16 mutants for single nucleotide polymorphisms. Neighbor-joining phylogenetic trees were constructed using E6, E7 and LCR sequences.
RESULTS
Fourteen missense mutations were found in the gene; the loci with the highest mutation frequency were T350G (36/75, 48%) and T178G (19/75, 25.3%). In the gene, the locus with the highest mutation frequency was A647G (18/75, 24%). A total of 33 polymorphic sites were found in the , of which T7447C (39/95, 40.1%) was the most frequent.
CONCLUSION
HPV16 in Xinjiang is mainly of the European variant, followed by the Asian variant type; no Africa 1, 2 or Asia-America variant types were found.
背景
新疆是中国宫颈癌发病率最高的地区之一。16型人乳头瘤病毒（HPV16）的基因变异可能会增强该病毒介导致癌作用和免疫逃逸的能力，而这是宫颈癌进展的危险因素。我们通过分析新疆HPV16阳性宫颈样本女性的E6、E7和长控制区（LCR）基因序列，研究了该地区HPV16的多态性及其亚谱系分布。
方法
共收集138例感染HPV16病毒的宫颈病变和鳞状细胞癌病例。对HPV16病毒的E6、E7基因和LCR进行测序，并与HPV16欧洲原型参考序列及其他HPV16突变体进行单核苷酸多态性比较。使用E6、E7和LCR序列构建邻接法系统发育树。
结果
在E6基因中发现14个错义突变；突变频率最高的位点是T350G（36/75，48%）和T178G（19/75，25.3%）。在E7基因中，突变频率最高的位点是A647G（18/75，24%）。在LCR中总共发现33个多态性位点，其中T7447C（39/95，40.1%）最为常见。
结论
新疆的HPV16主要为欧洲变异型，其次是亚洲变异型；未发现非洲1型、2型或亚洲-美洲变异型。
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